| 摘要: |
| 目的 研究血必净对脓毒症相关性血小板减少症(TCP)大鼠模型的肝脏是否具有保护作用。方法:选取雄性清洁级Sprague-Dawley 大鼠40 只，随机均分为4 组，假手术(Sham)组、盲肠结扎穿孔模型(CLP)组、血必净治疗(XBJ)组和血必净提前干预(pre-XBJ)组，每组各10 只。CLP 组、XBJ 组和pre-XBJ 组实施盲肠结扎穿孔术诱导脓毒症TCP 模型，Sham 组开腹不结扎穿孔；术前，pre-XBJ 组给予尾静脉注射血必净，Sham 组、CLP 组、XBJ 组给予等体积生理盐水；术后0 h、12 h，给予XBJ 组尾静脉注射血必净，其余各组给予等体积生理盐水；术后24 h，自大鼠眼球采血检测血小板计数，酶联免疫吸附法(ELISA)检测肝功能相关指标、炎症因子水平；HE 染色观察肝损伤及免疫组织化学检测肝脏中血小板滞留情况。结果:CLP 组大鼠血小板计数显著低于Sham 组，而pre-XBJ 组较CLP 组血小板计数增加(P<0.05)；XBJ 组和pre-XBJ 组谷丙转氨酶(ALT)、谷草转氨酶(AST)、总胆红素(TB)均较CLP 组降低(P<0.05)，炎症因子肿瘤坏死因子(TNF)-a、白细胞介素(IL)-6 和IL-18 表达量减少(P<0.05)；XBJ 组和pre-XBJ 组肺脏组织水肿、炎症细胞浸润及肝脏内血小板内滞留情况均较CLP 组减轻。结论:血必净可缓解脓毒症TCP 大鼠血小板计数减少，改善肝脏损伤及血小板在肝脏内聚集黏附，对脓毒症TCP 大鼠肝脏有一定的保护作用。 |
| 关键词: 血必净 脓毒症 血小板减少 盲肠结扎穿孔 大鼠 肝脏 |
| DOI：10.3969/j.issn.1007-6948.2025.01.003 |
| 投稿时间：2024-09-02 |
| 基金项目:天津市医学重点学科建设项目(TJYXZDXK-013A)；天津市卫生健康科技项目(ZC20012)；天津市科技计划项目(21JCYBJC00090、22JCQNJC00420) |
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| Research of the protective effect of Xuebijing on the liver of septic thrombocytopenic rats |
| LI Hong-jie,YANG Jun,XU Hua |
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| Abstract: |
| Objective To investigate the effect of Xuebijing on the liver injury in the septic thrombocytopenic rats. Methods Rats were randomly divided into four groups: control group (Sham), cecum ligation perforation model group (CLP), Xuebijing treatment group (XBJ) and Xuebijing prevent intervention group (pre-XBJ). The rats of CLP group，XBJ group and pre-XBJ group were performed cecal ligation and perforation to induce sepsis thrombocytopenia model and the Sham group rats were only opened without ligation and perforation. Before the operation, the pre-XBJ group rats were giving Xuebijing by tail vein injection and the other groups were giving equal volume of normal saline. The XBJ group rats were giving Xuebijing by tail vein injection at 0 h and 12 h after operation, and the other groups were given an equal volume of normal saline.Blood was collected from the abdominal aorta at the 24 h after operation to detect platelet counts, and ELISA was used to detect liver function related indicators, TNF-aand other inflammatory factors. HE staining was used to observe liver injury and immunohistochemistry to detect platelet retention in the liver. Results The platelet count of rats in the CLP group was significantly lower than that in the Sham group, while the platelet count of rats in the XBJ intervention groups increased than the CLP group (P<0.05). The AST，ALT and TB in the XBJ intervention groups were all lower than the CLP group (P<0.05), and the expression level of TNF-a, IL-6 and IL-18 were also reduced in the XBJ group and pre-XBJ group than that in the CLP group(P<0.05). The pulmonary tissue edema, inflammatory cell infiltration and platelet retention in the liver in the XBJ group and pre-XBJ group were all reduced compared with the CLP group. Conclusion Xuebijing can relieve the reduction of platelet count in septic thrombocytopenic rats, improve liver damage and platelet aggregation and adhesion in the liver, and has a certain protective effect on the liver of septic thrombocytopenic rats. |
| Key words: Xuebijing sepsis thrombocytopenic cecum ligation and puncture rat liver |
