| 摘要: |
| 目的:研究小金丸对大鼠阴茎硬结症模型阴茎海绵体内Toll样受体4(TLR4)、p38丝裂原活化蛋白激酶(MAPK)、基质金属蛋白酶(MMP)1的表达调节及对大鼠阴茎纤维化作用的可能机制。方法:将雄性SD大鼠18只随机分为3组:对照组、模型组、小金丸治疗组；对照组阴茎海绵体白膜下注射生理盐水，饲养6周后双蒸水灌胃；模型组、小金丸治疗组阴茎海绵体白膜下注TGF-β1(转化生长因子-β1)，饲养6周后用小金丸灌胃；模型组大鼠与小金丸治疗组等量双蒸水灌胃；3组灌胃处理4周后处死，留取大鼠阴茎组织。采用HE染色观察阴茎海绵体纤维化程度；PCR和Western Blot检测TLR4-p38MAPK-MMP1通路RNA和蛋白表达情况。结果:病理组织学HE染色发现，模型组比对照组纤维化明显增加；小金丸治疗组与模型组相比纤维化明显减少。对照组和小金丸治疗组的TLR4-p38MAPK-MMP1 RNA表达量及TLR4-p38MAPK-MMP1蛋白表达明显低于模型组，差异有统计学意义(P <0.01)。结论:阴茎硬结症大鼠模型阴茎海绵体内纤维化明显，TLR4-p38MAPK -MMP1表达增加；小金丸治疗后，纤维化明显改善，TLR4-p38MAPK-MMP1表达减少。小金丸治疗阴茎硬结症与TLR4-p38MAPK-MMP1介导的抗纤维化作用有关。 |
| 关键词: 阴茎硬结症 小金丸 TLR4-p38MAPK-MMP1信号通路 大鼠 抗纤维化 |
| DOI：10.3969/j.issn.1007-6948.2024.06.002 |
| 投稿时间：2024-05-15 |
| 基金项目: |
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| Antifibrotic mechanism of Xiaojin pills in the treatment of rat penile sclerosis |
| XU Yuan-ming,CHEN Shao-feng,ZHAO Feng |
| First Teaching Hospital of Tianjin University of Traditional Chinese Medicine/ National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300193, China |
| Abstract: |
| Objective To evaluate the effects and mechanism of Xiaojin pills for the treatment of a rat model of PD, and to research the level of TLR4-p38MAPK-MMP1 in the corpora cavernosa. Methods Twenty-four male Sprague-Dawley rats were randomly divided into three groups: control, PD and PD plus Xiaojin pills treatment. All rats underwent penile injections into the TA with 50 μL vehicle (control) or transforming growth factor (TGF)-β1 (50 μg/rats) injected into the TA (remaining groups). The PD group was gavaged 50 μL water twice one day on the 42 days after TGF-β1 injection. The PD plus Xiaojin pills treatment group was gavaged 50 μL solution (50 μg Xiaojin pills) twice one day on the 42 days after TGF-β1 injection). Twenty-eight days following intragastricing, the penile tissues of all rats were harvested and stored at 80 ℃ for further analysis. Tissues were evaluated histologically and for expression of TLR4-p38MAPK-MMP1. Results Pathological HE staining showed that the PD group had more obvious fibrosis than the control group. The fibrosis of Xiaojin pill treatment group was significantly improved compared with PD group. The expression of TLR4-p38MAPK-MMP1 RNA in control group and Xiaojin pill treatment group was significantly lower than that in PD group (P ＜0.01). The expression of TLR4-p38MAPK-MMP1 protein in control group and Xiaojin pill treatment group was significantly lower than that of PD group (P ＜0.01). Conclusions There was obvious fibrosis and increased expression of TLR4-p38MAPK-MMP1 in penile sponges of rats with penile sclerosis. After Xiaojin pill treatment, the fibrosis was significantly improved and the expression of TLR4-p38MAPK-MMP1 was reduced. The treatment of penile sclerosis with Xiaojin pills may be related to the anti-fibrosis mechanism mediated by TLR4- p38MAPK-MMP1. |
| Key words: Peyronie's disease Xiaojin pills TLR4- p38MAPK-MMP1 signal path rat antifibrosis |
