| 摘要: |
| 目的:探讨大承气汤对急性胰腺炎(AP)内分泌功能的影响及其可能机制。方法:采用腹腔注射雨蛙素诱导大鼠AP模型，在第一次注射雨蛙素前3 h和最后一次注射雨蛙素后9 h分别给予大承气汤灌胃。随后动态分析大鼠血糖和血清淀粉酶、胰岛素、C肽和胰高血糖素样肽-1(GLP-1)的水平，采用HE染色观察胰腺和回肠的组织病理学变化，采用免疫组化法检测回肠表达GLP-1的阳性细胞，并用ELISA法检测胰腺和回肠的GLP-1含量。结果:大承气汤灌胃可显著降低AP大鼠血清淀粉酶活性和血糖浓度，减轻胰腺和回肠组织病理学损伤，提高血清胰岛素、C肽、GLP-1水平和胰腺、回肠组织GLP-1水平，增加回肠GLP-1阳性细胞数量，并上调GLP-1蛋白表达水平。结论:大承气汤能改善AP大鼠胰腺内分泌功能，其机制与保护回肠L-细胞和促进内源性GLP-1分泌，进而增加胰岛素生成有关。 |
| 关键词: 大承气汤 胰高血糖素样肽-1 胰腺内分泌功能 急性胰腺炎 L-细胞 |
| DOI：10.3969/j.issn.1007-6948.2024.05.026 |
| 投稿时间：2024-07-01 |
| 基金项目:国家自然科学基金委员会(81202780)；天津市名中医王红工作室；国家中医药管理局胆胰病通里攻下重点研究室建设项目(2017-2019) |
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| The mechanism of Dachengqi decoction in improving endocrine pancreatic function in acute pancreatitis |
| QU Peng-fei,HAN Jun-quan,LI Cai-xia |
| Department of Surgery, the Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin (300151), China |
| Abstract: |
| Objective To investigate the effect of Dachengqi decoction (DCQD) on endocrine function and the potential mechanisms in acute pancreatitis (AP). Methods Rat AP models were induced by 6 intraperitoneal injections of caerulein in saline (50 μg/kg body weight), Dachengqi decoction (4.8 g/kg body weight) was orally given at 3 h before and 9 h after the last injection of caerulein. The blood glucose and serum amylase, the levels of insulin, C-peptide and glucagon-like peptide-1(GLP-1) were dynamically analyzed. The histopathological changes of pancreas and ileum, and the quantification of GLP-1 positive cells were observed. In addition, the GLP-1 levels in pancreas, ileum and ileal lumen were detected by ELISA. Results Dachengqi decoction treatment significantly reduced the serum amylase activity and blood glucose, alleviated the histopathological damage of pancreas and ileum. Moreover, Dachengqi decoction obviously increased the serum levels of insulin, C-peptide and GLP-1, as well as L-cells numbers of ileum in AP rats. Further determination indicated that Dachengqi decoction elevated the GLP-1 levels in the pancreas and ileum, decreased the GLP-1 level in ileal lumen. Conclusion Dachengqi decoction improves endocrine pancreatic function of AP, the mechanisms is related to protecting L-cells and raising the production of endogenous GLP-1, which in turn increases the level of insulin secreted by pancreas. |
| Key words: Dachengqi decoction glucagon-like peptide-1 endocrine pancreatic function acute pancreatitis L-cells |
