| 摘要: |
| 目的:采用网络药理学方法分析黄芪-当归(HQ-DG)治疗糖尿病足，促进溃疡愈合的作用机制。方法:检索中药系统药理学数据库与分析(TCMSP)数据库，查阅文献获得黄芪-当归潜在活性成分及作用靶点，通过GeneCards数据库、OMIM数据库、DDT数据库和Drugbank数据库获得糖尿病足的疾病潜在靶点；应用Metascape平台对潜在靶点进行基因本体(GO)富集分析和京都基因与基因组百科全书(KEGG)富集分析；运用String数据库和Cytoscape软件构建蛋白相互作用(PPI)网络和“药物-有效成分-靶点-通路”网络。结果:黄芪-当归治疗糖尿病足核心成分包括丁子香萜、异鼠李素、山柰酚、β-谷固醇/植物甾醇、豆甾醇等，核心靶点有基质金属酶(MMP)-9、血管内皮细胞生长因子A(VEGFA)、肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、CXCL8等，主要作用于AGE-RAGE糖尿病并发症信号通路、白细胞介素-17信号通路、TNF信号通路等途径，其功能主要为调节细胞增殖、迁移等。结论:本研究初步揭示了黄芪-当归治疗糖尿病足，促进溃疡愈合的多成分、多靶点、多通路的作用机制，为今后复方机制研究提供参考。 |
| 关键词: 黄芪-当归 网络药理学 糖尿病足 溃疡愈合 |
| DOI：10.3969/j.issn.1007-6948.2022.02.020 |
| 投稿时间：2021-05-18 |
| 基金项目:国家自然科学基金青年科学基金项目(81973854) |
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| Mechanism of Huangqi-Danggui on Diabetic Foot based on Network Pharmacology |
| NIU Wen-jing,LIU Peng,WANG Jun |
| First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, National Clinical Research Center of Chinese Medicine Acupuncture and Moxibustion,Tianjin (301600), China |
| Abstract: |
| Objective To analyze the action mechanism of Haungqi-Hanggui (HQ-DG) on diabetic foot. Methods To retrieve the TCMSP database and obtain the potential active ingredients of HQ-DG, potential disease targets for diabetic foot ulcers through GeneCards, OMIM, DDT and Drugbank databases. Gene ontology for potential targets using the Metascape platform (gene ontology, GO). Functional enrichment analysis and the Kyoto Encyclopedia of Gene and Genomics (Kyoto encyclopedia of genes and genomes, KEGG). The enrichment analysis building a protein interaction with the String Database and the Cytoscape Software (proteinprotein interaction, PPI) Network and “Drug - Composition - Target - Pathway”network. Results The core diabetes complication signaling, interleukin-17 signaling, TNF signaling pathway and etc. Its function are mainly components of HQ-DG for diabetic foot include MMP9, VEGFA, TNF, IL-6 and CXCL8, which mainly acts on AGE-RAGE proliferation and migration. Conclusion This study reveals the mechanism of HQ-DG in promoting the healing of diabetic foot ulcer via multi-component, multiple target and multiple pathway. It provides directional reference for future research of compound mechanism. |
| Key words: Huangqi-danggui network pharmacology diabetic foot ulcer wound healing |
