| 摘要: |
| 目的:研究异丙酚是否通过调控微小RNA(miRNA/miR)-506影响骨肉瘤细胞增殖、迁移侵袭及凋亡。方法:骨肉瘤HOS细胞分为NC组(未处理)、Pro-L组(1 μg/mL异丙酚)、Pro-M组(5 μg/mL异丙酚)、Pro-H组(10 μg/mL异丙酚)、miR-NC组(转染miR-NC)、miR-506组(转染miR-506 mimic)、Pro+anti-miR-NC组(转染antimiR-NC+10 μg/mL异丙酚)、Pro+anti-miR-506组(转染miR-506 inhibitor+10 μg/mL异丙酚)。应用细胞计数试剂盒8(CCK-8)法测定细胞增殖，免疫印迹实验(Western blotting)评估细胞周期蛋白(Cyclin)D1、p21、基质金属蛋白酶(MMP)-2、MMP-9、B细胞淋巴瘤/白血病-2(Bcl-2)、Bcl-2相关X蛋白(Bax)蛋白表达，Transwell实验检测迁移侵袭，流式细胞术分析细胞凋亡，荧光定量PCR测定miR-506表达。结果:与NC组比较，Pro-L、Pro-M、Pro-H组HOS细胞的抑制率、p21、Bax蛋白水平、凋亡率和miR-506表达水平逐渐增加，CyclinD1、MMP-2、MMP-9、Bcl-2蛋白水平、迁移细胞数和侵袭细胞数逐渐减少，且呈浓度依赖性，差异有统计学意义(P<0.05)。miR-506组HOS细胞的抑制率、凋亡率、p21、Bax蛋白水平比miR-NC组升高，迁移细胞数、侵袭细胞数、CyclinD1、MMP-2、MMP-9、Bcl-2蛋白水平比miR-NC组降低，差异有统计学意义(P<0.05)。Pro+anti-miR-506组较Pro+anti-miR-NC组降低HOS细胞中miR-506表达水平、抑制率、凋亡率、p21、Bax蛋白水平，提高迁移细胞数、侵袭细胞数、CyclinD1、MMP-2、MMP-9、Bcl-2蛋白水平，差异有统计学意义(P<0.05)。结论: 1、5、10 μg/mL异丙酚上调miR-506的表达，抑制骨肉瘤细胞增殖、迁移侵袭，并且促进其凋亡。 |
| 关键词: 异丙酚 miR-506 骨肉瘤 增殖 迁移侵袭 凋亡 |
| DOI：10.3969/j.issn.1007-6948.2021.05.002 |
| 投稿时间：2020-09-20 |
| 基金项目: |
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| Propofol Regulates the Effects of MiR-506 on the Proliferation, Migration, Invasion and Apoptosis of Osteosarcoma Cells |
| LIU Yuan-hao,ZHENG Yi-ming,WANG Bin |
| Department of Orthopedics, the Third People' s Hospital of Haikou, Hainan 571100, China |
| Abstract: |
| Objective To investigate whether propofol affects the proliferation, migration, invasion and apoptosis of osteosarcoma cells by regulating miR-506. Methods Osteosarcoma HOS cells were divided into NC group (untreated), Pro-L group (1 μg/mL propofol), Pro-M group (5 μg/mL propofol), Pro-H group (10 μg/mL propofol), miR-NC group (transfected with miR-NC), miR-506 group (transfected with miR-506 mimic), Pro+anti-miR-NC group (transfected with anti-miR-NC+10 μg /mL propofol) and Pro+anti-miR-506 group (transfected with miR-506 inhibitor+10 μg/mL propofol). Cell Counting Kit 8 (CCK-8) was used to measure cell proliferation. Western blotting was to evaluate CyclinD1, p21, MMP-2, MMP-9, Bcl-2, Bax protein expression and Transwell test to detect migration and invasion. Flow cytometry analyzed cell apoptosis, and fluorescence quantitative PCR was employed to determine the expression of miR-506. Results Compared with the NC group, the inhibition rate, p21, Bax protein level, apoptosis rate and miR-506 expression level of HOS cells in Pro-L, Pro-M and Pro-H groups gradually increased, while the protein level of CyclinD1, MMP2, MMP-9, Bcl-2, the number of migrating cells and the number of invasive cells gradually decreased, which showed a certain concentration-dependent, which the difference was statistically significant (P<0.05). The inhibition rate, apoptosis rate, p21 and Bax protein levels of HOS cells in the miR-506 group were higher than those in the miR-NC group, the number of migrating cells, the number of invading cells, CyclinD1, MMP-2, MMP-9 and Bcl-2 protein levels were lower than those of the miR-NC group, which the differences were statistically signi?cant (P<0.05). Compared with the Pro+antimiR-NC group, the Pro+anti-miR-506 group reduced the expression level, inhibition rate, apoptosis rate, p21 and Bax protein levels of miR-506 in HOS cells, and increased the number of migrating cells, the number of invasive cells, and the protein levels of CyclinD1, MMP-2, MMP-9 and Bcl-2, which the difference was statistically signi?cantly (P<0.05). Conclusions 1, 5, and 10 μg/mL propofol can up-regulate the expression of miR506, inhibit the proliferation, migration and invasion of osteosarcoma cells as well as promote their apoptosis. |
| Key words: Propofol miR-506 osteosarcoma proliferation migration and invasion apoptosis |
