| 摘要: |
| 目的:观察偎脓长肉法对大鼠皮肤慢性溃疡愈合过程中肉芽组织血管内皮细胞生长因子( VEGF)、转化生长因子β1(TGF-β1)及 Notch1表达的影响。方法:将 24只大鼠随机分为正常组、模型组、对照组、治疗组共 4组，每组 6只。正常组无创面，其他 3组大鼠采用“激素干预 -皮肤缺损 -细菌感染”复合因素叠加法制备慢性皮肤溃疡模型，模型组给予生理盐水换药，对照组给予凡士林纱条外敷，治疗组给予生肌象皮膏外敷，每日换药 1次，共 14 d。于治疗 3、7、14 d，分别取各组大鼠溃疡创面肉芽组织，行 HE染色观察组织病理学改变，并采用免疫组化方法检测 VEGF、TGF-β1、Notch1等的表达情况。结果:治疗组应用偎脓长肉法治疗 14 d后创面为(0.012±0.004)cm2，基本愈合，与模型组、对照组比较，差异有统计学意义( P<0.05)。在创面修复早、中期( 3~7 d)，与模型组、对照组比较，治疗组创面组织 TGF-β1、VEGF表达水平升高， Notch1表达水平降低，差异均有统计学意义( P<0.05)；疮面修复晚期( 14 d)，各指标差异无统计学意义(P>0.05)。结论:偎脓长肉法可能通过调节肉芽组织 VEGF、Notch1、TGF-β1的表达，起到促进肉芽组织生长、促进疮面修复的作用。 |
| 关键词: 药疮交互作用 偎脓长肉 慢性溃疡 生肌象皮膏 生长因子 |
| DOI：10.3969/j.issn.1007-6948.2020.01.002 |
| 投稿时间：2018-10-02 |
| 基金项目:天津市卫计委中医中西医结合基金项目(2015112)，天津市滨海新区卫计委科研项目(2018BWKY027)。 |
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| Effect of Weinongzhangrou Method on Expression of VEGF, Notch1 and TGFβ1 in Granulation Tissue of Rats with Chronic Ulcer and its Histopathology |
| ZHU Chao-jun,ZHANG Zhao-hui,ZHANG Yang |
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| Abstract: |
| Objective To observe the effects of Weinongzhangrou method on the expression of VEGF, Notch1 and TGF-β1 in the granulation tissue of rats with chronic ulcer. Methods Totally 24 rats were randomly divided into four groups: normal group, model group, control group and treatment group. The rats in normal group had no wound; The other three groups of rats used the compound factors superposition method of “hormone intervention-skin defect-bacterial infection” to duplicate the model of chronic skin ulcer. The rats in model group were given normal saline treatment, the rats in control group were given vaseline gauze external treatment, and the rats in treatment group were given external application of Shengji Xiangpi Plaster, once daily for 14 d. After 3, 7 and 14 d of treatment, the rat wound granulation tissues of rats were taken and stained by HE to observe the histopathological changes, and the expression of VEGF, TGF-β1 and Notch 1 were detected by immunohistochemistry. Results After treatment of 14 d, the wound healing in the treatment group was basically healed (0.012±0.004) cm2, and the difference was statistically signi.cant (P<0.05 or 0.01) compared with the model group and control group. In early and medium terms of wound healing (3 to 7 d), the expression levels of TGF-β1, VEGF and Notch 1 in the wound tissue of the treatment group increased compared with the model group and control group, but the difference was statistically significant (P<0.05); in the late stage of wound healing (14 d), there was no significant difference in each index (P>0.05) Conclusion Weinongzhangrou method may be used to regulate the expression of VEGF, Notch 1 and TGF-β1 in wound surface, which can promote the growth of granulation and wound healing. |
| Key words: Drug sore interaction Weinongzhangrou method chronic ulcer Shengji Xiangpi Plaster growth factor |
