| 摘要: |
| 目的：探讨黄芪甲苷（AS-IV）预处理对大鼠肠缺血再灌注损伤的影响。方法：30只成年雄性Wistar 大鼠随机分为假手术（Sham）组、模型（I/R）组、AS-IV预处理（AS-IV）组。I/R组和AS-IV组采用无创微动脉夹夹闭肠系膜上动脉60 min再灌注120 min的方法建立大鼠肠缺血再灌注损伤模型。AS-IV组于造模前60 min时采用60 mg/kg AS-IV对大鼠进行灌胃预处理。于再灌注120 min时取小肠组织，采用HE染色法测定小肠组织病理学变化，ELISA法测定大鼠血清中TNF-α、HMGB-1、SOD和CAT的表达情况，荧光定量 PCR法测定小肠组织中Bcl-2、Bax和Caspase-9的mRNA情况，Western blotting法测定小肠组织中紧密连接蛋白occludin和ZO-1的表达情况。结果：与Sham组比较，I/R组小肠组织病理学Chiu评分升高，TNF-α和HMGB-1的表达水平升高，SOD和CAT的表达水平降低，Bcl-2、Bax和Caspase-9的mRNA水平升高，occludin和ZO-1的蛋白表达水平降低（P<0.05）； 与I/R组比较，AS-IV组I/R组小肠组织病理学Chiu评分降低，TNF-α和HMGB-1的表达水平降低，SOD和CAT的表达水平升 高，Bcl-2、Bax和Caspase-9的mRNA水平降低，occludin和ZO-1的蛋白表达水平升高（P<0.05）。结论：AS-IV灌胃预处理能够显著改善大鼠肠I/R损伤，其机制可能与减轻机体炎症反应和氧化应激水平、抑制肠道细胞的凋亡发生以及调节紧密连接蛋白表达有关。 |
| 关键词: 缺血再灌注 肠损伤 黄芪甲苷 肠屏障 紧密连接 |
| DOI：10.3969/j.issn.1007-6948.2019.03.006 |
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| 基金项目: |
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| Effects of Astragaloside IV on Intestinal Ischemia-reperfusion Injury in Rats |
| REN Xiao-jing, YANG Tao, GENG Li-cheng |
| Department of Anesthesiology, Baodi District People' s Hospital of Tianjin, Tianjin(301800), China |
| Abstract: |
| Objective To investigate the effects of astragaloside A (AS-IV) pretreatment on intestinal ischemia-reperfusion (I/R) injury in rats. Methods Thirty adult male Wistar rats were randomly divided into Sham group, model (I/R) group and AS-IV pretreatment (AS-IV) group. Intestinal I/R model was induced via clamping the superior mesenteric artery for 60min, then followed by 120 min reperfusion in I/R group and AS-IV group. Rats received intragastric administration with 60 mg/kg AS-IV 60 min before ischemia in AS-IV group. After 120 min of reperfusion, pathological changes and score of the intestine were measured by HE staining; the levels of TNF-α, HMGB-1, SOD and CAT in serum were measured by ELISA assay; the mRNA levels of Bcl-2, Bax and Caspase-9 in intestinal tissues were measured by RT qPCR assay; the expression levels of occludin and ZO-1 were measured by Western blotting assay. Results Compared with Sham group, the Chiu score, levels of TNF-α and HMGB-1, and mRNA levels of Bcl-2, Bax and caspase-9 were increased, and levels of SOD and CAT and expression levels of occludin and ZO-1 were decreased in I/R group (P<0.05). Compared with I/R group, the Chiu score, levels of TNF-α and HMGB-1, and mRNA levels of Bcl-2, Bax and caspase-9 were decreased, and levels of SOD and CAT and expression levels of occludin and ZO-1 were increased in AS-IV group (P<0.05). Conclusion As-IV pretreatment can significantly alleviate intestinal I/R injury in rats, which is involved in lightening inflammation and oxidative stress, inhibiting apoptosis of intestinal cells, and regulating the expression of tight junction proteins. |
| Key words: Ischemia/reperfusion (I/R) intestinal injury astragaloside IV intestinal barrier tight junction |
