| 摘要: |
| 目的 探讨表没食子儿茶素没食子酸酯(EGCG)对胰腺癌细胞的增殖及糖代谢的影响。方法:用CCK8试剂盒检测不同浓度EGCG对胰腺癌PANC-1细胞增殖的影响，用葡萄糖乳酸试剂盒检测EGCG对糖酵解的影响，Western blot法检测糖酵解酶及PI3K/Akt通路蛋白的表达。结果:CCK8结果显示，EGCG可以抑制PANC-1细胞的增殖，其抑制作用具有剂量依赖性和时间依赖性(P＜0.05)。葡萄糖乳酸检测实验表明，EGCG可以抑制PANC-1细胞的葡萄糖消耗和乳酸生成(P＜0.05)。Western blot结果表明，EGCG可以抑制糖酵解酶己糖激酶-2(HK-2)、磷酸果糖激酶(PFK)和乳酸脱氢酶(LDH)及磷酸化PI3K和Akt的表达，且EGCG浓度越高，抑制作用越明显(P＜0.05)。结论:EGCG抑制人胰腺癌细胞系PANC-1的增殖和糖酵解，其抑制作用可能与抑制PANC-1细胞中PI3K/Akt通路有关。 |
| 关键词: 胰腺癌 表没食子儿茶素没食子酸酯 糖酵解 细胞增殖 PI3K/Akt通路 |
| DOI：10.3969/j.issn.1007-6948.2023.05.021 |
|
| 基金项目:天津市教育委员会自然科学类一般项目(2022KJ269) |
|
| EGCG Inhibits Glycolysis of Pancreatic Cancer Cells via PI3K/AKT Pathway |
| GAO Jing，WANG Feng，HU Li-juan |
|
| Abstract: |
| Objective To investigate the effects of epigallocatechin gallate (EGCG) on the proliferation and glucose metabolism of pancreatic cancer cells. Methods Human pancreatic cancer PANC-1 cells were treated with different concentrations of EGCG, and the proliferation of PANC-1 cells was assessed by CCK8 method. The effects of EGCG on PANC-1 cell glycolysis were detected by glucose consumption and lactate production, and the expressions of glycolytic enzyme and PI3K/Akt pathway protein were detected by Western blot. Results The results of CCK8 assay showed that EGCG inhibited the proliferation of pancreatic cancer PANC-1 cells in a dose- and time-dependent manner (P＜0.05). EGCG also inhibited the glucose consumption and lactate production of PANC-1 cells (P＜0.05). Compared with the control group, EGCG inhibited the expression of glycolytic enzyme and PI3K/Akt pathway protein in a dose-dependent manner (P＜0.05). Conclusion EGCG can inhibit the proliferation and glycolysis of human pancreatic cancer cells, which may be related to the inhibition of PI3K/Akt pathway in PANC-1 cells. |
| Key words: pancreatic cancer epigallocatechin gallate glycolysis cell proliferation PI3K/Akt pathway |
