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复方沙棘籽油加速大鼠肛周脓肿术后创面修复的作用机制研究
邵书先,王东,杨关根,裘建明,袁玥旻,何瑾
0
杭州市第三人民医院杭州 310009;杭州市中医院杭州 310009
摘要:
目的 探讨复方沙棘籽油加速大鼠肛周脓肿术后创面修复的作用机制。方法:取制备成功肛周脓肿术后创面SD模型鼠共50只,随机分为5组,分别为模型组(G1,给予蒸馏水);阳性对照组(G2,给予高锰酸钾);复方沙棘籽油纳米乳化液低剂量治疗组(G3);复方沙棘籽油纳米乳化液中剂量治疗组(G4);复方沙棘籽油纳米乳化液高剂量治疗组(G5);造模后各组实验大鼠创面用相应实验药物均匀涂抹,2次/d,连续给药14 d。观察治疗5 d、8 d、14 d后各组大鼠创面愈合情况,计算各组大鼠创面愈合率及分别取各组大鼠创面组织,应用免疫组化S-P法检测创面表皮生长因子(EGF)、磷酸化细胞外调节蛋白激酶(ERK)1/2、白细胞介素-8(IL-8)、前列腺素E2(PGE2)表达情况。结果:创面处理后5 d、8 d、14 d,G5组、G4组、G3组、G2组、G1组大鼠创面愈合率依次降低(均P<0.05) 。术后14 d大鼠创面IL-8表达水平为G2<G5<G4<G3<G1(P<0.05);PEG2表达水平为G5<G2<G4<G3<G1(P<0.05)。术后14 d大鼠创面EGF、ERK1/2表达为G5组> G4组> G3组> G2组> G1组 (均P<0.05)。结论:复方沙棘籽油纳米乳化液可有效促进大鼠肛周脓肿术后创面愈合,这可能与IL-8及PEG2炎症介质有关,还可能与“EGF-ERK”通路活化促进创面角质形成细胞、成纤维细胞生长、增殖、分化有关。
关键词:  复方沙棘籽油  肛周脓肿  创面修复  表皮生长因子  细胞外调节蛋白激酶
DOI:10.3969/j.issn.1007-6948.2023.05.019
基金项目:浙江省科技厅公益研究项目(LGF20H270001);杭州市医药卫生科技项目(A20200122)
Mechanism of compound seabuckthorn seed oil accelerating postoperative wound repair of perianal abscess in rats
SHAO Shu-xian,WANG Dong,YANG Guan-gen
Abstract:
Objective To study the effect of compound seabuckthorn seed oil on accelerating wound healing after perianal abscess surgery in rats. Methods A total of 50 SD model mice were used to successfully prepare postoperative wounds of perianal abscess. They were randomly divided into five groups: G1 model group (given distilled water); G2 positive control group (given potassium permanganate); G3 treatment group (low dose group of compound seabuckthorn seed oil nano-emulsion); G4 treatment group (middle dose group of compound seabuckthorn seed oil nano-emulsion); G5 (high dose group of compound seabuckthorn seed oil nano-emulsion). After modeling, the wounds of experimental rats in each group were evenly smeared with corresponding experimental drugs twice a day for 14 days. Observe the wound healing of rats in each group after 5 d, 8 d and 14 d of treatment, calculate the wound healing rate of rats in each group and take the wound tissue of rats in each group respectively, and analyze the expression of EGF, phosphorylated ERK1/2, IL-8 and PEG2 in the wound with immunohistochemical S-P method. Results The wound healing rate of rats in G5 group, G4 group, G3 group, G2 group and G1 group decreased in turn at 5 d, 8 d and 14d after wound treatment (all P<0.05). 14 days after surgery, IL-8 expression in rat wounds G2G4>G3>G2>G1 (all P<0.05). Conclusion Compound seabuckthorn seed oil nano-emulsion can effectively promote wound healing after perianal abscess surgery in rats, which may be related to the inflammatory mediators of IL-8 and PEG2, and its activation of "EGF-ERK" pathway can promote the growth, proliferation and differentiation of wound keratinocytes and fibroblasts.
Key words:  Compound seabuckthorn seed oil  perianal abscess  wound healing  epidermal growth factor  extracellular signal-regulated kinase

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