| 摘要: |
| 目的 探讨济川煎对慢传输型便秘大鼠模型的影响及其作用机制。方法:将40只SD大鼠随机分为正常组、模型组、济川煎高、中、低剂量组，每组8只。正常组予以10 mL/(kg·d)生理盐水灌胃14 d；其余组用浓度为1.5 mg/mL的复方地芬诺酯[10 mL/(kg·d)]灌胃14 d，建立STC模型。自第15天，济川煎低、中、高剂量组分别给予1.85 g/kg、3.7 g/kg、7.4 g/kg的济川煎灌胃至实验结束，正常组和模型组予以相同体积的蒸馏水。测定各组大鼠首粒黑便时间、肠道内碳末推进长度，用酶联免疫吸附试验(ELISA)法检测结肠组织Cajal间质细胞(ICC)的特异性标志物(C-Kit)和血清中血管活性肠肽(VIP)的含量。结果:与正常组相比，模型组大鼠首粒黑便时间显著增加、血清中VIP浓度明显上升、肠道碳末推进长度和结肠中C-Kit蛋白含量减少(均P＜0.05)。与模型组比较，济川煎低、中、高剂量组大鼠首粒黑便时间、血清中VIP浓度显著下降，肠道碳末推进长度和结肠C-Kit蛋白含量明显增加(均P＜0.05)；与济川煎中、低剂量组比较，济川煎高剂量组首粒黑便时间、血清VIP浓度显著降低，肠道碳末推进长度、结肠C-Kit含量显著升高，差异有统计学意义(P＜0.05)。结论:济川煎可促进肠道运动、改善便秘症状，其作用机制可能与增加结肠组织中C-Kit的表达、降低血清中VIP的含量有关。 |
| 关键词: 慢传输型便秘 济川煎 血管活性肠肽 C-Kit蛋白 |
| DOI：10.3969/j.issn.1007-6948.2023.05.018 |
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| 基金项目:天津市教委科研项目(2018KJ035) |
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| Investigation of the mechanism of Jichuan decoction intervention on serum VIP and colonic C-Kit to improve slow-transmission constipation in rats |
| QU Zi-yi，ZHANG Yu-tian，WANG Hong |
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| Abstract: |
| Objective To investigate the effect and mechanism of Jichuan decoction on rat models of slow-transit constipation. Methods Forty SD rats were randomly divided into normal group, model group, Jichuan fried high, medium and low dose group, with 8 rats in each group. The normal group was given 10 mL/(kg·d)normal saline gavage for 14 days; The remaining groups were gavaged with compound dipheNOxylate [10 mL/(kg·d)] at a concentration of 1.5 mg/mL for 14 days to establish an STC model. Jichuan decoc tion gavage of 1.85 g/kg, 3.7 g/kg and 7.4 g/kg was given to the low, medium and high dose groups of Jichuan decoction for 14 days, and the normal group and the model group were gavaged with the same volume of distilled water. The time of first melena and the length of carbon powder propagation in the colon were determined in each group. The ELISA method measures the content of C-Kit and serum vasoactive intestinal polypeptide(VIP) in colonic tissue. Results Compared with the normal group, the first melena time increased, the concentration of VIP in serum increased significantly, the length of carbon end propeller and the content of C-Kit protein in the colon decreased (all P<0.05). Compared with the model group, the first melena time and serum VIP concentration of rats in the low, medium and high dose groups in Jichuan fried significantly decreased, and the length of intestinal carbon powder advance and colonic C-Kit protein content increased significantly (all P<0.05), and compared with the Jichuan fried group, the first melena time and serum VIP concentration in the high-dose group were significantly reduced, the length of intestinal carbon powder advancement and colonic C-Kit content were significantly increased, and the difference between the medium and low dose groups was significantly higher (P<0.05). Conclusion Jichuan decoction can promote intestinal motility and improve constipation symptoms, and its mechanism of action may be related to increasing the expression of C-Kit in colon tissues and reducing the content of VIP in serum. |
| Key words: Slow-Transit Constipation Jichuan decoction vasoactive intestinal polypeptide C-Kit protein |
