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前列腺癌中丝氨酸苏氨酸激酶-1和驱动蛋白-5蛋白表达及相关性研究
罗荣利,徐旻,张春霆
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浙江大学医学院附属金华医院泌尿外科浙江金华 321000;浙江省金华市人民医院泌尿外科浙江金华 321000
摘要:
目的 检测前列腺癌中丝氨酸苏氨酸激酶-1(PIM-1)和驱动蛋白-5(Eg5)蛋白表达,探讨其表达与前列腺癌临床病理及它们之间的相关性。方法:采用免疫组化SP法检测81例前列腺癌和43例前列腺增生组织中PIM-1和Eg5蛋白表达水平,卡方检验分析PIM-1和Eg5蛋白表达阳性率与前列腺癌的临床病理特征之间的关系,采用Pearson相关分析PIM-1和Eg5蛋白在前列腺癌中的相关性。结果:PIM-1和Eg5蛋白在前列腺癌中表达增高,明显高于前列腺增生,差异有统计学意义(P<0.05),PIM-1和Eg5蛋白表达阳性率与Gleason评分、临床分期、术前PSA和5年生化复发有关(P<0.05);与患者年龄无关(P>0.05)。PIM-1和Eg5蛋白表达阳性率高的生化复发率高于PIM-1和Eg5蛋白表达阳性率低的患者 (P<0.05),Pearson相关性分析显示,前列腺癌中PIM-1和Eg5蛋白呈正相关(r=0.825,P<0.05)。结论:PIM-1和Eg5基因可能与前列腺癌演化和进展有关,二者联合检测有望提高对前列腺癌的预测能力。
关键词:  前列腺癌  丝氨酸苏氨酸激酶-1  驱动蛋白-5  免疫组织化学
DOI:10.3969/j.issn.1007-6948.2023.05.015
基金项目:浙江省基础公益研究计划项目资助(LGF20H050002)浙江省金华市重大科学技术研究计划项目资助(2021-3-027)
Expression and correlation of PIM-1 and Eg5 proteins in prostate cancer
LUO Rong-li,XU Min,ZHANG Chun-ting
Abstract:
Objective To detect the expression of serine threonine kinase-1(PIM-1) and kinesin-5 (Eg5) proteins in prostate cancer and to investigate their correlation with the clinicopathology of prostate cancer. Methods The expression of PIM-1 and Eg5 protein in 81 cases of prostate cancer and 43 cases of benign prostatic hyperplasia were detected by immunohistochemical streptavidin-perosidase method. The relationship between the positive rate of PIM-1 and Eg5 protein expression and the clinicopathological characteristics of prostate cancer were analyzed by chi square test. The correlation between PIM-1 and Eg5 protein in prostate cancer was evaluated by Pearson correlation analysis. Results The expressions of PIM-1 and Eg5 proteins in prostate cancer were significantly higher than those in benign prostatic hyperplasia (P<0.05). The positive rates of PIM-1 and Eg5 protein expressions were related to Gleason score, clinical stage, preoperative PSA and 5-year biochemical recurrence (P<0.05). They were not related to the patient's age (P>0.05). Biochemical relapse with high positive rate of PIM-1 and Eg5 protein expression was higher than that with low positive rate of PIM-1 and Eg5 protein expression (P<0.05). Pearson correlation analysis showed that PIM-1 and Eg5 proteins were positively correlated in prostate cancer (r=0.825, P<0.05). Conclusions PIM-1 and Eg5 genes may be related to the evolution and progression of prostate cancer, and joint detection may improve the prediction of prostate cancer.
Key words:  Prostate cancer  serine threonine kinase-1  kinesin-5  immunohistochemistry

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