Department of Pain, Wuqing Hospital of Ttraditional Chinese Medicine of Tianjin City, Tianjin (301700), China
Abstract:
Objective To investigate the regulated role of Nrf2 /HO- 1 on H 2 S alleviating the microglia activation in neuropathic pain in rats. Methods Sixty-four adult male Sprague-Dawley rats were randomly divided into four groups: control group (group Con, n = 16 ), neuropathic pain group (group SNL, n = 16 ), neuropathic pain+NaHS group (group SNL+NaHS, n = 16 ) and neuropathic pain+NaHS+Nrf 2 siRNA group (SNL+siRNA+NaHS, n=16). The animal model of neuropathic pain was performed by spinal nerve ligation (SNL). The L5-6 spine cords were collected for the detection of Nrf2 protein and Nrf2 mRNA by Western blotting and RT-PCR and IBA1 and Nrf2 co-location by immuno?uorescence after 7 d of administration in group Con, group SNL and group SNL+NaHS. After 1 , 2 , 3 , 5 and 7 d of operation, MWT and TWL were measured, and after detection of behavioristics, the L5-6 spine cords were collected for the detection of IBA1, Nrf2 and HO-1 protein by Western blotting and cytokines TNF-α and IL-1β by ELISA. Results Compared with group Con, MWT and TWL were reduced, Nrf2 nucleoprotein and mRNA, co-location of Nrf2 and IBA1 expression, total protein IBA1, Nrf2 and HO-1, and cytokines TNF-α and IL-1β were increased in group SNL; compared with group SNL, MWT and TWL were improved in group SNL+NaHS; compared with group SNL+NaHS, MWT and TWL were reduced, Nrf2 nucleoprotein and mRNA, the co-location of Nrf2 and IBA1 expression, total protein Nrf 2 and HO- 1 , were reduced, and total protein IBA1, cytokines TNF-α and IL-1β were increased in group SNL+NaHS+siRNA. Conclusion NaHS could alleviate the microglia activation by Nrf2/HO-1 pathway in neuropathic pain.
