Exploring the therapeutic effect and mechanism of Qingwen Badu decoction on sepsis-induced renal injury based on gut microbiota
WANG Cui-han,YANG Lei,WU Yu-lin
Abstract:
Objective: To investigate the therapeutic effect and mechanism of Qingwen Baidu(QWBD) decoction on septic kidney injury rats. Methods Fifty healthy male SD rats were randomly divided into five groups: Sham group, CLP group, QWBD low, medium, and high dose groups according to a random number table. Biochemical tests were conducted to detect infection indicators and liver and kidney function in the serum of the rats; HE observation of pathological changes in renal tissue; Immunohistochemical detection of MPO and Cit-H3 in rat kidney tissue; 16SrDNA sequencing was used to detect changes in gut microbiota. Results Compared with the CLP group, all dose groups of QWBD were able to reduce the infection index of rats to a certain extent and improve the damaged liver and kidney function of rats to a certain extent; The HE staining results of rat kidney tissue showed that the structure of the kidney tissue in the CLP group was severely damaged, and the dilation of renal small sacs gradually decreased in each dose group of QWBD. The watery degeneration of renal tubular epithelial cells gradually decreased, and the renal tubular epithelial cells gradually became round and full; Compared with CLP, the positive expression of MPO and Cit-H3 proteins in the renal tissue of rats in each dose group of QWBD was significantly reduced (P <0.01); The analysis of gut microbiota in each group of rats showed that in terms of alpha diversity, all dose groups of QWBD were able to increase Chao1, Simpson, and Shannon indices; In the beta diversity display, there were differences in gut microbiota between the CLP group and the Sham group, as well as differences between the QWBD group and both the CLP and Sham groups. The beta diversity was relatively high; Species composition heatmap analysis suggests an increase in beneficial bacteria and a decrease in harmful bacteria after QWBD intervention. At the phylum, class, order, family, and genus levels, it suggests that the CLP group has significantly more harmful bacteria, while the QWBD group has significantly more beneficial bacteria, indicating that QWBD can significantly improve gut microbiota. Conclusion QWBD has a clear therapeutic effect on septic kidney injury rats, and its therapeutic mechanism may be related to regulating gut microbiota and inhibiting NETs.
