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经乙二醇诱导的改良型草酸钙肾结石果蝇模型
曹益方,赵凡,石兵
0
浙江省嘉兴市第一医院泌尿外科 /嘉兴学院附属医院泌尿外科浙江嘉兴 314001;江苏省南通大学附属医院泌尿外科江苏南通 226001;江苏省南通市中医院泌尿外科 /南京中医药大学南通附属 医院泌尿外科江苏南通 226001
摘要:
目的:通过一种改良方法构建果蝇草酸钙肾结石模型。方法:配制标准培养基和造模用培养基,空白对照组果蝇仅予标准培养基,而传统模型组和改良模型组分别从成虫期和幼虫期开始摄入含 0.5%乙二醇( EG)的造模用培养基。于偏光显微镜下观察并评估各组果蝇马氏管内成石情况,分别记录模型组成石率达 100%所花时间,并对成石率 100%时模型组间“ ++”和“ +++”所占比率进行比较;用傅立叶变换拉曼光谱仪鉴定模型组果蝇马氏管内结石成分。绘制各组果蝇生存曲线,并比较生存周期差异。结果:改良模型组和传统模型组分别在果蝇成虫日龄 14 d和 22 d,马氏管内成石率达到 100%。当改良模型组和传统模型组成石率均达到 100%时,两组“ ++”与“+++”所占比率分别为(40.5±4.4)%和(39.0±4.2)%,差异无统计学意义(P> 0.05);拉曼位移的主峰主要集中在 1 462 cm-1、1 463 cm-1和 1 473 cm-1,说明模型组果蝇马氏管内结石成分均为草酸钙。空白对照组、传统模型组和改良模型组的最高寿命分别为 76 d、70 d和 68 d,中位生存时间分别为 35 d、30.5 d和 30 d,与空白对照组相比,模型组生存周期均显著缩短(P均< 0.01),但传统模型组与改良模型组间差异无统计学意义( P> 0.05)。结论:改良型造模方法使果蝇在其幼虫期即摄入 0.5% EG,缩短了模型构建周期且具有可重复性,值得进一步研究推广。
关键词:  乙二醇  草酸钙  肾结石  果蝇  动物模型
DOI:10.3969/j.issn.1007-6948.2022.03.003
投稿时间:2021-10-24
基金项目:嘉兴市医学重点学科建设计划(泌尿外科学,2019-zc-07);嘉兴市重点实验室(嘉兴市泌尿系肿瘤精准诊治重点实验室,2020-mnzdsys)
Improved Drosophila Model for Nephrolithiasis of Calcium Oxalate Induced by Ethylene Glycol
CAO Yi-fang,ZHAO Fan,SHI Bing
Abstract:
Objective To establish Drosophila calcium oxalate (CaOx) nephrolithiasis model via an improved method. Methods Standard medium and modeling medium were prepared. The blank control group of Drosophila was only given standard medium, while the traditional model group and the improved model group were fed with modeling medium containing 0.5% ethylene glycol (EG) from adult stage and larval stage respectively. The stone formation in Drosophila martensis tube under polarizing microscope was observed and evaluated, the time spent when the stone formation rate of the model reaches 100% was recorded, and the proportion of“++”and“+++” between the model groups when the stone formation rate reached 100% were compared. The Fourier transform Raman spectroscopy was used to identify the components of stones in Drosophila martensis tube in the model group. The survival curve of Drosophila in each group was drawn, and the
Key words:  Ethylene glycol  calcium oxalate  nephrolithiasis  drosophila  animal model

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