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miR-7-5p通过靶向抑制成纤维生长因子受体 4影响胆囊癌细胞的增殖和迁移
李云超,孙占峰,苏彬,胡金朋,王振国,王月明
0
天津市宁河区医院普外科天津 301500;武警特色医学中心研究部天津 300162
摘要:
目的:探讨miR-7-5p对胆囊癌细胞增殖和迁移的影响及作用机制。方法:选取胆囊癌细胞GBC-SD,检测miR7-5p和成纤维生长因子受体4(FGFR4)表达情况;通过TargetScan数据库检索调控FGFR4的miRNA,并用双重荧光素酶基因检测系统检测FGFR4与miR-7-5p结合情况;通过慢病毒将miR-7-5p转染GBC-SD细胞,检测FGFR4、miR-7-5p mRNA和FGFR4蛋白表达,MTT检测细胞活性,Transwell检测细胞侵袭。结果:在GBC-SD细胞中miR-7-5p低表达且FGFR4高表达(P<0.05);TargetScan预测FGFR4特异结合miR-7-5p,并在双重荧光素酶基因检测系统得到验证;GBCSD细胞慢病毒转染miR-7-5p后,FGFR4 mRNA和蛋白表达水平下调,细胞增殖率下降,穿膜细胞数减少,差异有统计学意义(P<0.05);沉默miR-7-5p后,FGFR4 mRNA和蛋白表达水平上升,细胞增殖率升高,穿膜细胞数增加,差异有统计学意义(P<0.05)。结论: miR-7-5p通过靶向抑制FGFR4而抑制胆囊癌细胞的增殖和迁移。
关键词:  胆囊癌  微小RNA-7-5p  成纤维生长因子受体4  GBC-SD细胞
DOI:10.3969/j.issn.1007-6948.2022.03.001
投稿时间:2021-08-13
基金项目:天津市科技计划项目(16ZXHLSY00120、15ZXLCSY00040);武警部队后勤部科研项目(CWJ18L004)
miR-7-5p Affects Proliferation and Migration of Gallbladder Carcinoma Cells by Targeting FGFR4 Inhibition
LI Yun-chao,SUN Zhan-feng,SU Bin
Abstract:
Objective To investigate the effect and mechanism of miR-7-5p on the proliferation and migration of gallbladder cancer cells. Methods GBC-SD cells were selected to detect the expression of miR-7-5p and fibroblast growth factor receptor 4 (FGFR4). The miRNA regulating FGFR4 was retrieved from TargetScan database, and the binding of FGFR4 to miR-7-5p was detected by dual luciferase gene detection system. The miR-7-5p was transfected into GBC-SD cells by lentivirus to detect the expression of FGFR4, miR7-5p mRNA and FGFR4 protein, cell activity by MTT and cell invasion by Transwell. Results The expression of miR-7-5p was low and FGFR4 was high in GBC-SD cells (P<0.05). TargetScan predicted that FGFR4 specifically bound miR-7-5p and was verified in the dual luciferase gene detection system. After lentivirus transfection of miR-7-5p into GBC-SD cells, the expression levels of FGFR4 mRNA and protein decreased, the cell proliferation rate decreased and the number of transmembrane cells decreased (P<0.05). After silencing miR-7-5p, the expression levels of FGFR4 mRNA and protein increased, the cell proliferation rate increased and the number of transmembrane cells increased, with statistical significance (P<0.05). Conclusion miR-7-5p inhibits the proliferation and migration of gallbladder cancer cells by targeting FGFR4.
Key words:  gallbladder carcinoma  microRNA-7-5p  fibroblast growth factor receptor 4  GBC-SD cell

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