| 摘要: |
| 目的:探讨miR-7-5p对胆囊癌细胞增殖和迁移的影响及作用机制。方法:选取胆囊癌细胞GBC-SD，检测miR7-5p和成纤维生长因子受体4(FGFR4)表达情况；通过TargetScan数据库检索调控FGFR4的miRNA，并用双重荧光素酶基因检测系统检测FGFR4与miR-7-5p结合情况；通过慢病毒将miR-7-5p转染GBC-SD细胞，检测FGFR4、miR-7-5p mRNA和FGFR4蛋白表达，MTT检测细胞活性，Transwell检测细胞侵袭。结果:在GBC-SD细胞中miR-7-5p低表达且FGFR4高表达(P＜0.05)；TargetScan预测FGFR4特异结合miR-7-5p，并在双重荧光素酶基因检测系统得到验证；GBCSD细胞慢病毒转染miR-7-5p后，FGFR4 mRNA和蛋白表达水平下调，细胞增殖率下降，穿膜细胞数减少，差异有统计学意义(P＜0.05)；沉默miR-7-5p后，FGFR4 mRNA和蛋白表达水平上升，细胞增殖率升高，穿膜细胞数增加，差异有统计学意义(P＜0.05)。结论: miR-7-5p通过靶向抑制FGFR4而抑制胆囊癌细胞的增殖和迁移。 |
| 关键词: 胆囊癌 微小RNA-7-5p 成纤维生长因子受体4 GBC-SD细胞 |
| DOI：10.3969/j.issn.1007-6948.2022.03.001 |
| 投稿时间：2021-08-13 |
| 基金项目:天津市科技计划项目(16ZXHLSY00120、15ZXLCSY00040)；武警部队后勤部科研项目(CWJ18L004) |
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| miR-7-5p Affects Proliferation and Migration of Gallbladder Carcinoma Cells by Targeting FGFR4 Inhibition |
| LI Yun-chao，SUN Zhan-feng，SU Bin |
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| Abstract: |
| Objective To investigate the effect and mechanism of miR-7-5p on the proliferation and migration of gallbladder cancer cells. Methods GBC-SD cells were selected to detect the expression of miR-7-5p and fibroblast growth factor receptor 4 (FGFR4). The miRNA regulating FGFR4 was retrieved from TargetScan database, and the binding of FGFR4 to miR-7-5p was detected by dual luciferase gene detection system. The miR-7-5p was transfected into GBC-SD cells by lentivirus to detect the expression of FGFR4, miR7-5p mRNA and FGFR4 protein, cell activity by MTT and cell invasion by Transwell. Results The expression of miR-7-5p was low and FGFR4 was high in GBC-SD cells (P<0.05). TargetScan predicted that FGFR4 specifically bound miR-7-5p and was verified in the dual luciferase gene detection system. After lentivirus transfection of miR-7-5p into GBC-SD cells, the expression levels of FGFR4 mRNA and protein decreased, the cell proliferation rate decreased and the number of transmembrane cells decreased (P<0.05). After silencing miR-7-5p, the expression levels of FGFR4 mRNA and protein increased, the cell proliferation rate increased and the number of transmembrane cells increased, with statistical significance (P<0.05). Conclusion miR-7-5p inhibits the proliferation and migration of gallbladder cancer cells by targeting FGFR4. |
| Key words: gallbladder carcinoma microRNA-7-5p fibroblast growth factor receptor 4 GBC-SD cell |
