| 摘要: |
| 目的:观察紫黄生肌膏对大鼠慢性感染性创面血管生成及血管内皮生长因子(VEGF)和血管生成素(Ang)-1表达的影响。方法:将84只SD大鼠随机分为4组，分别是空白组、模型组、贝复济组、紫黄生肌膏组，造模成功后，除空白组外，其余组每天换药前在创面上涂粪液1 mL，保留30 min后予以清洁换药，于用药后3、7、14 d观察创面的大体愈合情况，同时在第3、7、14天取创面肉芽组织观察毛细血管生长数量，并用ELISA法检测创面肉芽组织中的VEGF、Ang-1的含量。结果:紫黄生肌膏大鼠创面渗血渗液较少，肉芽组织生长良好，在第7和14天，创面愈合速度明显快于模型组及贝复济组，差异有统计学意义(P<0.01)；在第7和14天，与模型组、贝复济组相比，紫黄生肌膏组创面肉芽组织中新生毛细血管数量明显增多，差异有统计学意义(P<0.05)，组织中VEGF、Ang-1的含量亦显著增加，差异有统计学意义(P<0.01)。结论:紫黄生肌膏对大鼠慢性感染性创面修复有明显的促进作用，能缩短创面愈合时间，促进血管生成，其机制可能与其快速有效上调VEGF、Ang-1有关。 |
| 关键词: 紫黄生肌膏 慢性感染性创面 血管内皮生长因子 血管生成素-1 |
| DOI：10.3969/j.issn.1007-6948.2022.02.006 |
| 投稿时间：2021-04-21 |
| 基金项目:苏州市科技发展计划(民生科技－医疗卫生应用基础研究)项目(SYS2019107)；苏州市中医医院院内课题(YQN20108004) |
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| Effect of Zihuang Shengji Ointment on Angiogenesis and Expression of VEGF and Ang-1 in Chronic Infection Wound |
| YANG Xiao,WU Ben-sheng,HE Zong-qi |
| Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou 215009, China |
| Abstract: |
| Objective To investigate the molecular mechanism of Zihuang Shengji Ointment in promoting angiogenesis of chronic infectious wounds. Methods 84 SD rats were randomly divided into 4 groups: blank group, model group, rb-bFGF group, Zihuang Shengji Ointment group. After successfully establishing chronic infectious wound models, all groups were treated with 1 mL of fecal fluid on the wound surface and kept for half an hour before clean dressing everyday, in addition to blank group. The effect of wound healing was observed at 3, 7 and 14 days after treatment, and the number of capillaries was observed too. The expressions of VEGF and Ang-1 were detected by ELISA. Results Zihuang Shengji Ointment had less blood and fluid in the wound, and the granulation tissue grew well. On the 7th and 14th day, a large number of new blood vessels were found in the wound, and the wound healing was significantly faster than that in the model group and rb-bFGF group (P<0.01). On the 7th and 14th day, compared with the model group and rb-bFGF group, VEGF and Ang-1 in Zihuang Shengji Ointment group were significantly increased (P<0.01). Conclusion Zihuang Shengji Ointment can significantly promote wound healing, shorten wound healing time and promote angiogenesis in rats with chronic infectious wounds. Its mechanism may be related to the rapid and effective up-regulation of VEGF and Ang-1. |
| Key words: Zihuang Shengji Ointment chronic infectious wound VEGF Ang-1 |
