| 摘要: |
| 目的:观察糖尿病大鼠与正常大鼠在创伤愈合过程中不同时期创面炎症相关因子的动态变化的差异性，探讨影响糖尿病溃疡愈合的因素。方法:通过腹腔注射链脲佐菌素和外科方法建立糖尿病大鼠全层皮肤缺损模型，设立糖尿病溃疡模型组和正常组，并运用免疫荧光和免疫组化方法检测M1、M2型巨噬细胞和白细胞介素(IL)-1、IL-6、IL-10、肿瘤坏死因子(TNF)-α的水平变化。结果:炎症期糖尿病溃疡模型组创面M1型巨噬细胞数量明显较少，增殖期糖尿病溃疡模型组创面M2型巨噬细胞数量明显较少；与正常组不同，糖尿病溃疡模型组增殖期IL-1β[(7.169±0.785)%]高于炎症期[(6.038±0.939)%]，差异有统计学意义(P<0.05)；TNF-α[(8.651±0.646)%]高于炎症期[(4.860±0.654)%]，差异有统计学意义(P<0.01)；IL-6水平[(9.627±1.211)%]高于炎症期[(5.457±0.643)%]，差异有统计学意义(P<0.01)，而IL-10上升幅度较低。结论:糖尿病性溃疡延迟愈合的原因可能与巨噬细胞表型和转化异常，以及促炎因子和抑炎因子间的动态失衡有关。 |
| 关键词: 糖尿病溃疡 炎症因子 创面愈合中图分类号: R641 文献标识码: A 文章编号: 1007-6948(2021)05-0693-06 |
| DOI：10.3969/j.issn.1007-6948.2021.05.004 |
| 投稿时间：2021-02-04 |
| 基金项目:上海中医药大学高峰高原创新项目(A1-N19205 01010212) ；国家自然科学基金项目(81973580，81774317)；上海市中医药领军人才学术共同体建设项目( ZYSNXD-RC-LJRC)；上海市海派中医流派(顾氏外科)传承研究基地建设项目(ZY3CCCX-1-1004)；龙华医院科技创新项目(KY1773) |
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| Differential Study on Dynamic Changes of Inflammatory Factors in Diabetic Rats and Normal Rats at Different Stages of Wound Healing |
| ZHANG Zhen,QUE Hua-fa |
| Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China |
| Abstract: |
| Objective To observe the differences in dynamic changes of wound inflammation-related factors in diabetic rats and normal rats at different stages during wound healing, and to explore the influence factors of the healing of diabetic ulcers. Methods By intraperitoneal injection of Streptozocin and surgical method to establish the rat model of diabetes ulcers. The different expressions of various detection indexes were observed and compared including macrophages, interleukins, TNF-αof diabetic model and normal control groupby the methods of immunohistochemistry. Results The number of M1-type macrophages of diabetic ulcer model group was significantly lower in the inflammatory stage, while the number of M2-type macrophages was significantly lower in the regenerated stage. It is different from the normal control group that the levels of IL-1 of the diabetic ulcer model group inthe regenerated stage[(7.169±0.785)%]were significantly higher than those in the inflammatory stage[(6.038±0.939)%] (P<0.05).The levels of TNF-αof the diabetic ulcer model group in the regenerated stage[(8.651±0.646)%]were significantly higher than those in the inflammatory stage[(4.860±0.654)%] (P<0.01). The levels of IL-6 of the diabetic ulcer model group inthe regenerated stage[(9.627±1.211)%]were significantly higher than those in the inflammatory stage[(5.457±0.643)%] (P < 0 . 01 ) while the ascensional range of IL-10 were significantly smaller than those of the normal group. Conclusion The reasons of delayed healingof diabetic ulcer may be related to abnormal phenotype and transformation of macrophage, and the dynamic imbalance between proin?ammatory factors and anti-in?ammatory factors. |
| Key words: Diabetic ulcer in?ammatory factors wound healing |
