| 摘要: |
| 目的:探讨右美托咪定复合舒芬太尼鞘内注射对坐骨神经慢性缩窄性损伤(CCI)模型大鼠DRG神经元GABAA激活电流的作用。方法:鞘内置管成功的雄性SD大鼠50只，随机分成5组:假手术组(Sham组)、模型对照组(CCI组)、右美托咪定组(Dex组)、舒芬太尼组(Suf组)、右美托咪定+舒芬太尼组(DS组)。术后1~14天每天鞘内注射，Dex组注射右美托咪定(Dex)2 μg/kg、Suf组注射舒芬太尼(Suf)1 μg、DS组注射Dex 1 μg/kg+Suf 0.5 μg；各组药物总容量均配成20 μL Sham组、CCI组注射等体积0.9％生理盐水。每组分别在术前、术后1、3、7、14 天给药30 min后，测定机热痛阈(TWL)和械痛阈(MWT)。于术后第7天痛阈值测定后，提取大鼠L4、6节段脊髓(n=9)，应用全细胞膜片钳技术检测大鼠背根神经GABA激活电流(IGABA)。结果:与Sham组相比，CCI组、Dex组、Suf组及DS组术后3、7、14天机械痛阈和热痛阈显著降低(P<0.05)；与CCI模型组相比，Dex组、Suf组及DS组在术后3、7、14天机械痛阈和热痛阈显著升高(P<0.05)；与DS组相比，Dex组及Suf组在术后3、7、14天TWL及MWT阈显著降低(P<0.05)。术后7天与CCI模型组相比，Dex组、Suf组及DS组IGABA水平在显著增强(P<0.05)；与DS组相比，Dex组及Suf组IGABA水平显著减弱(P<0.05)。结论:鞘内联合应用右美托咪定及舒芬太尼治疗神经病理性疼痛具有显著的协调镇痛作用，并且这种协同镇痛作用与调节GABA激活电流有关。 |
| 关键词: 神经病理性疼痛 鞘内注射 右美托咪定 舒芬太尼 背根神经节 γ-氨基丁酸 |
| DOI：10.3969/j.issn.1007-6948.2021.04.016 |
| 投稿时间：2021-04-05 |
| 基金项目:国家自然科学基金(81301131) |
|
| Effects of Dexmedetomidine and Sufentanil Combined Intrathecally Injection on GABAA Activation Current in DRG Neurons of CCI Model Rats |
| SHU Luo-wa,WANG Gu-yan |
| Department of Anesthesiology, Capital Medical University Affiliated Beijing Tongren Hospital, Beijing 100730, China |
| Abstract: |
| Objective To investigate the analgesic effect of combined dexmedetomidine and sufentanil intrathecal injection on CCI model rats. Methods Intrathecal catheters were successfully inplanted in 50 male SD rats.Fifty male SD rats with intrathecal catheters were randomly divided into 5 groups(n=10 per group): sham group(Sham), model control group(CCI group), dexmedetomidine group(Dex group), sufentanil group(Suf group) and combined treatment group(DS group). Intrathecal injection was given 1 to 14 days after operation. The rats in Dex group was injected with dexmedetomidine 2μg/kg. The rats in Suf group were injected in with sufentanil 1μg/d, The rats in DS group were injected in with dexmedetomidine1μg/kgandSufentanil 0.5μg. The total capacity of each group of drugs was diluted into 20μL. The rats in sham group and CCI group were intrathecally injected with an equal volume of 0.9% saline daily. The paw thermal withdrawal latency(TWL)and mechanical withdrawal threshold (MWT) were measured 30 min after intrathecal injection preoperative 1 day and 1, 3, 7, 14 days after CCI the 7th day after the operation. The TWL and MWT were measured, and the dorsal root ganglion cells of L4 to 6 rats were extracted andisolated (n = 9 per group). Whole cellpatch clamp technique was used to detect GABAactivation current in rat dorsal root cells. Results Compared with sham group, the TWL and MWT in CCI group, Dex group, Suf group and DS group were significantly decreased on the3rd day, the 7thday and the 14thday after surgery (P<0.05).Compared with CCI group, the TWL and MWT in Dex group, Suf group, DS group, and DS group were signi?cantly increased on the 3rdday, the 7th day and the 14th day after CCI (P<0.05). Compared with DS group, the TWL and the MWT in Dexgroup and Suf group were signi?cantly decreased on the 3rd day, the 7thday and 14th day after CCI (P<0.05). Compared with CCI model group, IGABA in Dex group, Suf group and DS groups were signi?cantly increased at 7 days after operation (P<0.05). Compared with DS group, IGABA in Dex group and Suf group were signi?cantly decreased (P<0.05). Conclusion Combined intrathecal application of dexmedetomidine and sufentanil in the treatment |
| Key words: Neuropathic pain intrathecal injection dexmedetomidine sufentanil dorsal root ganglion |
