| 摘要: |
| 目的:观察活血通降方对反流性食管炎(RE)大鼠模型食管黏膜NF-κB/MAPK信号通路的影响，探讨中药治疗RE的作用机制。方法:将40只大鼠随机平均分为对照组、造模组、西药组、中药组4组。对照组不处理，正常饲养；余3组采用改良部分贲门肌切开加外置幽门部分结扎术进行RE造模。术后14 d，造模组予0.9%生理盐水灌胃，西药组予西药悬浊液(奥美拉唑、铝碳酸镁及多潘立酮)灌胃，中药组予中药混悬液(活血通降方)灌胃，均治疗14 d。灌胃第7、14天，各组处死半数大鼠，观察食管下端黏膜大体及病理评分变化，并进行p38MAPK、NF-κBP65免疫组化染色，取血清检测白细胞介素(IL)-6及肿瘤坏死因子(TNF)-α浓度。结果:治疗第7天，西药组和中药组食管黏膜大体及病理评分降低，与造模组相比差异显著(P>0.05)，西药组和中药组差异无统计学意义(P>0.05)；治疗第14天，西药组和中药组食管黏膜大体及病理评分进一步降低，与造模组相比，差异更显著(P<0.01)，与对照组相比差异无统计学意义(P>0.05)，西药组和中药组差异无统计学意义(P>0.05)。造模后，造模组与对照组对比，p38MAPK及NK-κB免疫组化染色、血清IL-6及TNF-α表达显著升高(P>0.01)，治疗第7、14天，西药组和中药组上述检测内容表达低于造模组，差异有统计学意义(P<0.05)；但与对照组相比差异无统计学意义(P>0.05)，西药组和中药组间差异无统计学意义(P>0.05)。结论:活血通降方可以有效改善食管黏膜炎症反应，抑制p38MAPK/NF-κB炎症信号通路，减少炎性因子的产生，通过控制RE免疫炎症可治疗RE，且疗效与治疗时间呈正相关。 |
| 关键词: 反流性食管炎 活血通降方 p38MAPK 核因子-κB 炎性因子 |
| DOI：10.3969/j.issn.1007-6948.2021.04.002 |
| 投稿时间：2020-12-02 |
| 基金项目:国家自然科学基金(82074213，81573737) |
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| Effect of Huoxuo Tongjiang Prescription on NF-κB/MAPK Signal Pathway of Esophageal Mucosa in Rats with RE |
| LIU Lei,TANG Yan-ping,GONG Yan-xia |
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| Abstract: |
| Objective The aim of this study was to investigate the effect of Huoxue Tongjiang prescription on the NF-κB/MAPK signal pathway of esophageal mucosa in rats model of RE, and explore the mechanism of traditional Chinese medicine in the treatment of RE furtherly. Methods 40 rats were randomly divided into 4 groups: control group, model group, western medicine group and Chinese medicine group. The control group was fed without treatment; the other 3 groups were treated with RE (the method was modified partial cardiotomy and external partial pylorus ligation). After operation, the model group was given 0.9% normal saline for 14 days, and the western medicine group was given western medicine suspension (domperidone+ omeprazole+ aluminum magnesium carbonate), and the Chinese medicine group was given Huoxue Tongjiang prescription, all for 14 days. The general conditions of the rats were observed. On the 7th and 14th day, half of the rats in each group were killed. The pathological score, p38MAPK and NF-κB p65 immunohistochemical staining were observed and the serum levels of IL-6 and TNF-α were measured. Results On the 7th day of treatment, the scores of esophageal mucosa in western medicine group and Chinese medicine decreased significantly compared with those of the model group (P<0.05), indicating that western medicine and Chinese medicine have curative effects on RE model rats, but there is nostatistical difference between western medicine group and traditional Chinese medicine (P > 0.05).On the 14th day of treatment, the general specimens of esophageal mucosa in western medicine group and traditional Chinese Medicine decreased. Compared with the model group, the difference was more signi?cant (P < 0.01). Compared with the control group, there was no statistical difference (P > 0.05). There was no statistical difference between the western medicine group and Chinese medicine group (P > 0.05). Compared with the control group, the expression of p38MAPK and NK-κB inflammatory pathway, IL-6 and TNF - α in the serum increased signi?cantly (P < 0.01). On the 7th day of treatment, the expression of the above-mentioned indexes decreased significantly in the western medicine group and the Chinese medicine, which compared with the model group (P < 0.05). On the 14th day of treatment, there was no significant differencethe expression of the above-mentioned indexes between the western medicine group and the Chinese medicine group(P > 0.05). Conclusion Huoxue Tongjiang prescriptiontreatment can effectively improve the inflammation and histopathological changes of esophageal mucosa, inhibit the p38MAPK / NF-κB inflammatory signal pathway, reduce the production of inflammatory factors and treat RE by controlling the immune inflammation of RE.The effect is positively correlated with treatment time. |
| Key words: Re?ex esophagitis Huoxue Tongjiang prescription p38MAPK NF-κB in?ammatory factors |
