| 摘要: |
| 目的:探讨 MicroRNA-133(miR-133)在胆管癌细胞系中的表达，研究 miR-133基因表达在胆管癌细胞迁移和侵袭过程中的作用。方法:利用实时荧光定量 PCR(RT-PCR)技术测定分析 miR-133及其不同成熟体( miR-133b、miR133a-3p、miR-133a-5p)在胆管癌 QBC939细胞系中的表达。将胆管癌 QBC939细胞系经转染后分为三组:自然生长组(空白对照组)，细胞转染无关序列组(阴性对照组)以及细胞转染 Anti-miR-133a-5p组(干扰组)，利用 RT-PCR检测各组胆管癌 QBC939细胞系中 miR-133a-5p、c-met的表达。然后利用 Transwell小室法分别检测分析干扰 miR-133a-5p基因表达对胆管癌 QBC939细胞系迁移和侵袭能力的影响。结果: RT-PCR检测 miR-133不同成熟体 miR-133b(0.74±0.07)、miR133a-3p(0.99±0.12)、miR-133a-5p(1.00±0.06)中的表达，其中 miR-133a-5p在胆管癌 QBC939细胞系中表达量最高(P<0.05)。RT-PCR实验证实干扰组 QBC939细胞系中 miR-133a-5p(0.70±0.08)表达显著低于对照组( 1.43±0.34)；干扰组 QBC939细胞系中 c-met(0.09±0.02)表达也显著低于对照组( 1.01±0.07)，差异有统计学意义( P<0.01)。 Transwell小室实验证实干扰组中胆管癌 QBC939细胞系迁移( 69.5±8.3)和侵袭( 19.3±3.3)能力明显低于对照组，差异均有统计学意义( P<0.01)。结论: MicroRNA-133a-5p的低表达可以明显减弱 c-met的表达，同时可以抑制胆管癌 QBC939细胞系的迁移和侵袭能力，miR-133a-5p有可能成为胆管癌基因表达调控的新靶点。 |
| 关键词: 微小 RNA-133 胆管癌 c-met 迁移 侵袭 |
| DOI：10.3969/j.issn.1007-6948.2020.03.006 |
| 投稿时间：2019-08-13 |
| 基金项目:北京医卫健康公益基金会医学科学研究基金资助项目(B17134) |
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| The Effect of MicroRNA-133a-5p on the Migration and Invasion of Cholangiocarcinoma Cells Through Regulatingc-met |
| YANG Bo,LIU Xiao-fang |
| Department of Hepatobiliary-Pancreatic Surgery, Affiliated YantaiYuhuangding Hospital, Qingdao University, Yantai264000,China |
| Abstract: |
| Objective To investigate the expression of MicroRNA-133 (miR-133) in cholangiocarcinoma cells and to study the role of miR-133 gene expression in the migration and invasion of cholangiocarcinoma cells. Methods Real-time PCR (RT-PCR) was used to analyze the expression of miR-133 and its different matures(miR-133b、miR-133a-3p、miR-133a-5p) in cholangiocarcinoma cells QBC939. QBC939 cells were chosen and divided into three groups: natural growth (blank control group), cellgroups transfection with negative sequence (negative control group) and cell groups transfection with anti-miR-133a-5p(interference group). The expression of miR-133a-5pand c-met in the QBC939 cells of each group were measured by RT-PCR. The effect of miR-133a-5p on the migration and invasion of QBC939 cells was analyzed by Transwell assay. Results The expression of miR-133b(0.74±0.07), miR-133a-3p (0.99±0.12) and miR-133a-5p (1.00±0.06) in miR-133 was detected by RT-PCR,miR-133a-5p had the highest expression in cholangiocarcinoma cells QBC939(P<0.05). RT-PCR experiments confirmed that the expression of miR-133a-5p (0.70±0.08) in the interference group QBC939 cells was significantly lower than that in the negative control group (1.43±0.34, P<0.05) and the expression of c-met (0.09±0.02) in the interference group QBC939 cells was signi?cantly lower than that in the blank control group ( 1 . 01 ± 0.07, P<0.01). Transwell experiments confirmed that the migration (69.5 ± 8.3) and invasion (19.3 ± 3 . 3 ) abilities netof QBC939 cells in the interference group were signi?cantly lower than that of the negative and blank control groups (both P<0.01). Conclusion The low expression of microRNA-133a-5p can signi?cantly attenuate the expression of c-met and inhibit the migration and invasion of cholangiocarcinoma cells QBC939. The miR-133a-5pmaybecome a new target for the regulation of cholangiocarcinoma gene expression |
| Key words: MicroRNA-133 cholangiocarcinoma c-met migration invasion |
