| 摘要: |
| 目的:研究下肢静脉性溃疡患者血清中基质金属蛋白酶 -3(MMP-3)、基质金属蛋白酶抑制剂 -1(TIMP-1)及超敏 -C反应蛋白( hs-CRP)的表达水平，探讨公英解毒洗剂治疗下肢静脉性溃疡的机制。方法:选取下肢静脉性溃疡患者 75例，随机分为对照组 25例，复方黄柏液组 25例，公英解毒洗剂组 25例。于治疗的第 1、7、14天抽取患者足背静脉血，检测血清中 MMP-3、TIMP-1及 hs-CRP表达情况并比较，计算并比较三组的创面愈合率。结果:治疗第 7天，创面愈合率和 TIMP-1由高到低依次为:公英解毒洗剂组 >复方黄柏液组 >对照组，差异均有统计学意义；治疗第 14天，公英解毒洗剂组和复方黄柏液组的创面愈合率和 TIMP-1均高于对照组，差异有统计学意义，但公英解毒洗剂组与复方黄柏液组差异无统计学意义。治疗第 7天，血清中 hs-CRP和 MMP-3水平由高到低依次为:对照组 >复方黄柏液组 >公英解毒洗剂组，差异均有统计学意义；治疗第 14天，复方黄柏液组和公英解毒洗剂组的血清 hs-CRP和 MMP-3均低于对照组，差异有统计学意义，但公英解毒洗剂组与复方黄柏液组差异无统计学意义。结论:通过对创面的观察及 MMP-3、TIMP-1和 hs-CRP表达的动态监测，证实公英解毒洗剂有较强的抗菌消炎的功效，值得临床推荐。 |
| 关键词: 下肢静脉性溃疡 公英解毒洗剂 基质金属蛋白酶 -3 基质金属蛋白酶抑制剂 -1 |
| DOI：10.3969/j.issn.1007-6948.2020.01.010 |
| 投稿时间：2019-02-21 |
| 基金项目:济南市科技局资助项目(合同号: 201705063)；陈柏楠山东省名老中医药专家传承工作室 |
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| Effect of Gongying Detoxification Lotion on Expression of MMP-3 and TIMP-1 in Venous Ulcer of Lower Extremity |
| ZHAO Bo,WANG Yan-nan,XU Yong-kai |
| Department of Peripheral Vascular Disease, the Af.liated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250014, China |
| Abstract: |
| Objective Based on the expression of MMP-3 and TIMP-1 in the venous ulcer of the lower extremity, to explore the therapeutic mechanism and target of Gongying Detoxification Lotion (GDL) in the treatment of venous ulcer of lower extremity. Methods In clinical studies, 75 patients with venous ulcer of lower limbs were selected. They were randomly divided into 3 groups: control group (n=25), Fufang Huangbai Liquid (FHL) group (n=25) and GDL group (n=25). On the 0, 7th, and 14th day, the blood of the dorsal vein of the foot was extracted and the expression of MMP-3 and TIMP-1 was detected by ELISA. The relevant experimental data were measured and the expression of hs-CRP was detected. Results On the 7th day of wound observation in the clinical study group, the detection results of hs-CRP and MMP-3 in serum showed that the expression of hs-CRP and MMP-3 in the three groups at the early stage of in.ammatory response increased, among which the expression of hs-CRP and MMP-3 in the control group was signi.cantly higher than that in the GDL group and the FHL group (P < 0.01), and the expression of hs-CRP and MMP-3 in the serum of the GDL group and the FHL group was also signi.cantly different (P < 0.05). On the 14th day of wound observation in the clinical study group, the expression of hs-CRP and MMP-3 in the serum of GDL group and FHL group was still significantly different from that of the control group (P <0.05). Conclusions The balance regulation mechanism of MMP-3 and TIMP-1 was not only involved in the local inflammatory reaction of ulcer wound but also expressed in the stage of cell proliferation, differentiation and tissue reconstruction wound healing. |
| Key words: Venous ulceration of lower extremity Gongying Detoxification Lotion matrix metalloproteinase-3 matrix metalloproteinase inhibitor-1 |
