| 摘要: |
| 目的：考察黄柏碱对脓毒症大鼠急性肾损伤的保护作用及其机制。方法：取 50 只 SD 大鼠随机分为假手术组、模型组、黄柏碱低剂量组（100 mg/kg）、黄柏碱高剂量组（200 mg/kg）及氨苄青霉素钠组（150 mg/kg），每组 10 只。除假手术组外，各组采用盲肠结扎穿孔术（CLP）建立脓毒症大鼠模型。术后 24 h，试剂盒检测血清肌酐（Cr）和尿素氮（BUN）的水平，ELISA 法检测血清中性粒细胞明胶酶相关脂质运载蛋白（NGAL）、肾损伤分子 1（KIM-1）、白细胞介素 -6（IL-6）、白细胞介素 -1β（IL-1β）和肿瘤坏死因子 -α（TNF-α），HE 染色法检测肾脏病变情况，免疫印迹法检测肾组织中 Toll样受体 4(TLR4) 及 Nod 样受体蛋白 3(NLRP3) 蛋白的表达。结果：与脓毒症大鼠比较，黄柏碱可明显降低大鼠血清中 Cr、BUN、NGAL 及 KIM-1 含量，抑制炎性因子（IL-6、IL-1β及 TNF-α）的释放，改善肾脏病理变化，同时可明显降低肾组织中 TLR4 及 NLRP3 的表达水平（P < 0.05）。结论：黄柏碱可通过调控 TLR4/NLRP3 信号通路降低体内炎症因子水平，从而对脓毒症所致急性肾损伤发挥保护作用。 |
| 关键词: 黄柏碱 脓毒症 急性肾损伤 炎症 TLR4/NLRP3 信号通路 |
| DOI：10.3969/j.issn.1007-6948.2019.06.004 |
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| 基金项目: |
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| Effect and Mechanism of Phellodendrine on Acute Kidney Injury in Sepsis Rats |
| JIANG Ying-ying,LIN Yue |
| Department of Emergency, Wenzhou People' s Hospital, Wenzhou (325000), China |
| Abstract: |
| Objective To investigate the protective effect and mechanism of phellodendrine on acute kidney injury in sepsis rats. Methods Fifty SD rats were randomly divided into sham operation group, model group, phellodendrine low dose and high dose group (100 and 200 mg/kg), and ampicillin sodium group (150 mg/kg). Except the sham operation group, the sepsis rats were established by cecal ligation and perforation (CLP). After 24 h of surgery, the serum creatinine (Cr), urea nitrogen (BUN), neutrophil enzyme related lipid carrier protein gelatin molecule 1 (NGAL), renal injury (KIM-1), interleukin 6 (IL-6), interleukin 1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) were detected. HE staining was used to detect the kidney disease situation. WB method was used to detect the toll-like receptor 4 (TLR4) and Nod like receptor protein 3 (NLRP3) protein expression in renal tissue. Results Compared with the sepsis rats, the contents of Cr, BUN, NGAL and KIM-1 in serum of the rats were signi?cantly reduced by phellodrine, which also inhibited the release of in?ammatory factors (IL-6, IL-1β and TNF-α), improved renal pathological changes, and signi?cantly reduced the expression levels of TLR4 and NLRP3 in renal tissues, (P < 0.05). Conclusion Phellodendrine can regulate TLR4/NLRP3 signaling pathway and reduce the levels of in?ammatory factors in vivo, so as to play a protective role in acute kidney injury caused by sepsis. |
| Key words: Phellodendrine sepsis acute renal injury inflammation TLR4/NLRP3 signaling pathway |
