| 摘要: |
| 目的：研究复方黄黛片对 Solt-Farber 原发性肝癌大鼠血管新生作用的影响。方法：将 50 只 SD 大鼠随机分为正常组、模型组、复方黄黛片高剂量组、中剂量组及低剂量组，每组 10 只。采用 Solt-Farber 二步法对除正常组外各组大鼠进行原发性肝癌造模，高（280 mg/kg）、中（140 mg/kg）及低剂量组（70 mg/kg）大鼠同时灌胃给予相应剂量的复方黄黛片，正常组与模型组则给予等量生理盐水，连续灌胃 18 周。末次灌胃 12 h 后取血，分离血清，检测其中肝功能指标水平（ALT、AST 及 TBIL）。取血完成，取各组大鼠肝脏，平均分为 4 份，分别进行病理组织学观察，采用免疫荧光法检测 HIF-1α、 VEGF 及 VEGFR-2 的表达情况，计算其相对表达量并进行 4 组比较。结果：与模型组 [ALT,（163.74±16.45）IU/L ；AST,（331.24±31.35）IU/L ；TBIL,（86.11±8.51）IU/L] 比较，各组大鼠中血清肝功能指标均明显降低，其差异具有统计学意义（P<0.05）；各组指标分别为高剂量组 [ALT,（71.43±7.83）IU/L ；AST,（112.52±12.58）IU/L ；TBIL,（34.23±3.23）IU/L]，中剂量组 [ALT,（105.32±10.13）IU/L ；AST,（223.52±36.58）IU/L ；TBIL,（42.93±4.02）IU/L]，低剂量组 [ALT,（122.34±12.11）IU/L ；AST,（254.21±25.14）IU/L ；TBIL,（51.27±5.26）IU/L]。高剂量组、中剂量组及低剂量组大鼠肝组织均出现不同程度的癌变，癌细胞呈梁状排列，但癌变程度均不及模型组。与模型组（HIF-1α，14.23±1.28 ； VEGF，11.52±1.10 ；VEGFR-2，16.34±1.65）比较，各组大鼠肝组织 HIF-1α、VEGF 及 VEGFR-2 的阳性表达情况均明显减轻，且相对表达量均明显降低，其差异具有统计学意义（P<0.05）；各组指标分别为高剂量组（HIF-1α，3.92±0.29 ；VEGF，2.87±0.26 ；VEGFR-2，3.45±0.40）、中剂量组（HIF-1α，6.36±0.65 ；VEGF，5.34±0.51 ；VEGFR-2，5.87±0.60）及低剂量组（HIF-1α，8.53±0.81 ；VEGF，7.76±0.74 ；VEGFR-2，8.12±0.87）。结论：复方黄黛片用于 Solt-Farber 原发性肝癌大鼠，可有效改善肝功能，抑制 HIF-1α、VEGF 及 VEGFR-2 等血管新生相关蛋白的表达。 |
| 关键词: 复方黄黛片 Solt-Farber 原发性肝癌 血管新生 大鼠 |
| DOI：10.3969/j.issn.1007-6948.2019.06.003 |
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| 基金项目: |
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| Effect of Fufang Huangdai Tablets on Solt-Farber Primary Liver Cancer in Mice and Its Influence on Angiogenesis Effects |
| TI Zhen-yu,,GAO Xiao-peng,,QIAN Dong-wei |
| Second Department of General Surgery, Third Hospital of Xi' an, Xi' an (710018), China |
| Abstract: |
| Objective To observe the effects of Fufang Huangdai Tablets (FHT) on Solt-Farber primary liver cancer in mice and its in?uence on the angiogenesis effects. Methods Totally 50 Sprague-Dawley mice were divided into 5 groups, such as control group, model group, high dose group, medium dose group and low dose group. The models were made by Solt-Farber method in the groups except the model group. The mice in high dose group, middle dose group and low dose group were treated by FHT ( 280g, 140 and 70 mg/kg), the treatment continued for 18 weeks. After 12 h of the last treatment, the serum of mice was obtained and the livers were isolated. The levels of ALT, AST and TBIL were detected in the serum and the pathological and histological were also observed in the groups. The fluorescent-immunohistochemistry was performed in the groups to observe the expression of HIF- 1 α,VEGF and VEGFR- 2 . Results Compared with model group [ALT, ( 163 . 74 ± 16 . 45 ) IU/L; AST,(331.24±31.35) IU/L; TBIL, (86.11±8.51) IU/L],the serum liver function indexes of the rats in eachgroup were signi?cantly reduced, and the differences were statistically signi?cant (P < 0. 05). The indicators of each group were high dose group [ALT, (71.43±7.83) IU/L; AST, (112.52±12.58) IU/L; TBIL, (34.23±3.23)IU/L]; medium dose group [ALT, (105.32±10.13) IU/L; AST, (223.52±36.58) IU/L; TBIL, (42.93±4.02)IU/L], and low dose group [ALT, (122.34±12.11) IU/L; AST, (254.21±25.14) IU/L; TBIL, (51.27±5.26)IU/L]. Compared with the model group, the liver pathological histology showed that the canceration degrees were significantly promoted. Compared with model group (HIF- 1 α, 14 . 23 ± 1 . 28 ; VEGF, 11 . 52 ± 1.10; VEGFR-2, 16.34±1.65); the positive expression of HIF-1α, VEGF and VEGFR-2 in liver tissues of rats in each group were signi?cantly reduced and the relative expression was signi?cantly decreased, with statistically significant differences (P < 0 . 05). The indicators of each group were high dose group (HIF-1α, 3 . 92± 0.29; VEGF, 2.87±0.26; VEGFR-2, 3.45±0.40), medium dose group (HIF-1α, 6.36±0.65; VEGF, 5.34±0.51;VEGFR- 2 , 5 . 87 ± 0 . 60 ) and low dose groups (HIF- 1 α, 8 . 53 ± 0 . 81 ; VEGF, 7 . 76 ± 0 . 74 ; VEGFR- 2 ,8.12 ± 0 . 87 ). Conclusion FHT on Solt-Farber method induced primary liver cancer in mice has a good protective effect, and could inhibit the expression of HIF-1α, VEGF and VEGFR-2. |
| Key words: Fufang Huangdai Tablets Solt-Farber method primary liver cancer angiogenesis rats |
