| 摘要: |
| 目的：研究黄芪多糖（astragaluspolysacharin，APS）对人脐静脉内皮细胞（human umbilical vein endothelial cells，HUVEC）周期及血管内皮细胞生长因子（vascular endothelial growth factor，VEGF）表达的影响。方法：用不同浓度黄芪多糖对 HUVEC 进行干预培养，流式细胞仪检测细胞周期以及细胞凋亡；荧光倒置显微镜检测细胞表达 VEGF 的免疫荧光。结果：MTT 法表明，APS 在一定的范围内（0.1~100 μg/mL）能以剂量依赖方式促进 HUVEC 细胞周期从 G0/G1 期向 G2/M 期和 S 期转变，流式细胞凋亡检测显示 APS 并未增加 HUVEC 凋亡率。细胞免疫荧光染色实验结果表明，APS 促进 VEGF 表达。结论：APS 在一定的浓度范围内（0.1~100 μg/mL）促进 HUVEC 细胞分裂增殖。APS 还能上调 HUVEC 细胞 VEGF 的表达水平，为进一步血管化提供有效的促血管化因子，为治疗缺血性疾病提供新思路。 |
| 关键词: HUVEC 细胞黄芪多糖血管化 血管内皮细胞生长因子 |
| DOI：10.3969/j.issn.1007-6948.2019.06.001 |
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| 基金项目:浙江省中医药科技计划科研基金项目（2015ZA173）；浙江省医药卫生科技计划项目（2015126904）；杭州市科技计划引导项目（20163501Y77）；萧山区重大科技专项（2015204） |
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| Effects of Astragalus Polysaccharides on Proliferation and Expression of VEGF in Human Umbilical Vein Endothelial Cells |
| HE Li-hong,ZHENG Xuan,MO Jia-hang |
| Xiaoshan Hospital of Traditional Chinese Medicine of Hangzhou City, Hangzhou (311201), China |
| Abstract: |
| Objective To explore the effect of Astragalus polysaccharide (APS) on the cell cycle of human umbilical vein endothelial cells (HUVEC) and the expression of vascular endothelial growth factor (VEGF). Methods HUVEC were cultured with different concentration of APS, and the cell cycle and apoptosis were detected by flow cytometry. Immunofluorescence staining of VEGF in cells was detected by fluorescence microscope. Results MTT assay showed that APS promoted the cell cycle of HUVEC from G0/G1 phase to G2/M phase and S phase within a certain concentration range. Flow cytometry apoptosis assay showed that APS did not increase the apoptosis rate of HUVEC. Cellular immuno?uorescence staining showed that APS promoted VEGF expression. Conclusion APS could promote division and proliferation of HUVEC at a certain concentration range (0.1–100 μg/mL). In addition, APS can also up-regulate the expression level of VEGF in HUVEC, providing an effective angiogenic factor for further vascularization and a new idea for the treatment of ischemic diseases. |
| Key words: Human umbilical vein endothelial cells astragaluspolysacharin vascularization vascular endothelial growth factor |
