| 摘要: |
| 目的：观察补肝健腰方对大鼠腰椎间盘突出症模型神经功能评分及 IL-1 表达的影响。方法：取 SD 大鼠 100 只， 随机分为正常对照组、假手术组、模型对照组、补肝健腰方组、塞来昔布组各 20 只，成功制备大鼠腰椎间盘突出模型，分别给与补肝健腰方、塞来昔布灌胃，检测各组干预前、干预第 1、2、3 周神经功能评分及 IL-1 表达，并进行对比。结果： 干预前，与模型对照组比较，补肝健腰方组、塞来昔布组腰椎间盘突出局部组织 IL-1 表达及神经功能评分无统计学差异（P>0.05）。补肝健腰方灌胃后第 1、2、3 周，补肝健腰方组 IL-1 表达分别为 19.29±0.61、14.54±0.23、14.01±0.21，均较模型对照组下降（P<0.05），同时，补肝健腰方组灌胃后第 1、2、3 周神经功能评分分别为 2.60±0.55、2.40±0.5、2.20±0.84，均较模型对照组下降（P<0.05）；补肝健腰方组灌胃后第 1、2、3 周以上指标表达均较干预前下降（P<0.05）。结论：补肝健腰方可降低大鼠腰椎间盘突出症模型突出部位 IL-1 的表达，改善神经功能评分，这可能为其治疗腰椎间盘突出症的作用机制。 |
| 关键词: 补肝健腰方 大鼠 腰椎间盘突出症 白介素 1 神经功能评分 |
| DOI：10.3969/j.issn.1007-6948.2019.05.003 |
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| 基金项目:国家中医药管理局“全国名老中医药专家仇湘中传承工作室建设项目”资助 ( 国中医药人教 [2016]42 号 ) ；湖南省中医药科研计划重点项目（201710）；湖南省自然科学基金面上项目（2017JJ2170）；湖南省中医药研究院重点课题（201503） |
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| Influence of Bugan Jianyao Decoction on IL-1 Expression and Neurological Function Score in Rat Lumbar Disc Herniation |
| TAN Xu-yi,ZHAO Di-min,QIU Xiang-zhong,CHOU Jie,Jiang Sheng-chang,ZHANG Xin-cheng,TANG Hao |
| Hunan University of TCM, Changsha (410208), China |
| Abstract: |
| Objective To observe the in?uence of Bugan Jianyao Decoction (BJD) on IL-1and neurological function score in rat lumbar disc herniation. Methods Totally 100 rats were randomly divided into normal control group, sham-operated group, model control group, BJD group and Celecoxib group, each group with 20 rats.After rat lumbar disc herniation model was prepared successfully, BJD group were gived the BJD intragastric administration, while Celecoxib group was given Celecoxib, normal control group, Sham-operated group, model control group were intragastrically administrated with the same dose of normal saline. IL- 1 expression and neurological function score were detected at the moment of the experimental intervention, the intervention of the 1, 2, 3 weeks. Results At the moment of the intervention,compared with the model control group,there was no statistically signi?cant that IL-1 expression and neurological function score in lumbar intervertebral disc local tissue of BJDgroup and Celecoxib group (P>0.05).After the intervention of the 1, 2, 3 weeks by BJD, IL-1 expression of BJDgroup were 19.29±0.61, 14.54±0.23, 14.01±0.21 respectively, were decreased than that of model control group (P<0.05),meanwhile, neurological function score of BJDgroup were 2.60±0.55, 2.40±0.5,2.20±0.84 respectively, were decreased than that of model control group (P < 0 . 05),after the intervention of the 1 , 2 , 3 weeks. Meanwhile, IL- 1 expression and neurological function score of BJDgroup were decreased than that at the moment of the intervention when after the intervention of the 1 , 2 , 3 weeks (P <0.05). Conclusions Bugan Jianyao Decoction has the effect of reducing IL-1 expression, improving neurological function score, it may be a mechanism for the treatment of lumbar disc herniation. |
| Key words: Buganjianyao Decoction rat lumbar disc herniation IL-1 neurological function score |
