| 摘要: |
| 目的：探讨结直肠癌组织中驱动蛋白超家族中 18A（KIF18A）的表达及其与患者临床病理特征和预后的关系。方法：采用实时荧光定量 PCR 方法，检测 35 例患者结直肠癌组织及相应癌旁正常组织（距肿瘤边缘大于 5 cm）中 KIF18A mRNA 的表达水平；回顾性分析前期接受手术治疗的 92 例结直肠癌患者的临床病理特征和术后随访资料，采用免疫组织化学方法检测结直肠癌组织标本和 20 例正常结直肠组织（非癌旁组织）中 KIF18A 蛋白的表达情况；比较不同病理特征患者的 KIF18A 的表达率，并分析其表达水平与结直肠癌患者预后的关系。结果：35 例结直肠癌组织中 KIF18A mRNA 表达量为 4.056±0.4024，明显高于相应癌旁正常组织的 1.253±0.1438（P<0.01）。KIF18A 主要表达在细胞质，KIF18A 蛋白在结直肠癌组织中的高表达率 80%（74/92），显著高于正常结直肠组织中高表达率 15%（3/20，χ2= 32.741，P<0.01）。KIF18A 在结直肠癌组织中的表达水平与肿瘤浸润深度（χ2= 15.031，P<0.01）、淋巴结转移（χ2= 6.064，P<0.05）及 pTNM 分期（χ2= 6.546，P<0.05）有关。KIF18A 高表达组 3 年生存率为 54.1%，低表达组为 100%（χ2=15.326,P<0.01）。单因素 Cox 回归分析显示，肿瘤大小（P<0.05）、分化程度（P<0.01）、浸润深度（P<0.01）、淋巴结转移情况（P<0.01）、 p TNM 分期（P<0.01）及 KIF18A 表达水平（P<0.01）与预后相关。多因素 Cox 回归分析显示，KIF18A 表达水平是影响结直肠癌预后的独立危险因素（HR =11.419, 95% CI:1.504~86.684, P<0.05）。结论：KIF18A 在结直肠癌组织中表达水平较高，提示其可能与结直肠癌患者的不良预后有关，有可能作为结直肠癌诊治和预后的潜在标志物。 |
| 关键词: 驱动蛋白超家族 18A 结直肠肿瘤 预后 |
| DOI：10.3969/j.issn.1007-6948.2019.04.002 |
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| 基金项目:浙江省嘉兴市科技技术局科技计划项目（2015AY23027） |
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| Expression of KIF18A in Colorectal Carcinoma and its Relationship with Clinicopathological Features |
| LI Bin,FENG Lian-zhong,WANG Chun-hua,BAO Tie,ZHENG Li,DONG Lai-rong |
| The Second Affiliated Hospital of Jiaxing University, Jiaxing (314000), China |
| Abstract: |
| Objective To explore the expression of KIF18A in colorectal carcinoma tissues and its relationship with various clinicopathological parameters and prognosis of the patients. Methods The fresh paired colorectal cancer tissues and adjacent normal tissues (> 5 cm from the edge of tumor) from 35 colorectal cancer patients undergoing operation from Jan. 2016 to Jun. 2016 were collected. The relative expression of KIF18A at mRNA level was detected by real-time fluorescence quantitative Polymerase Chain Reaction. Clinicopathological and follow-up data of 92 patients with colorectal carcinoma undergoing operation from Jan. 2012 to Jul. 2012 were retrospectively analyzed. The expression of KIF18A protein in samples of 92 resected colorectal cancer tissues and 20 normal colorectal tissues was detected by immunohistochemisty. The expression rates of KIF18A were compared among different clinicopathological features. Moreover, the association between KIF18A expression and prognosis was analyzed. Results The relative expression quantity of KIF18A at mRNA level in colorectal cancer tissues (4.056±0.4024) was signi?cantly higher than that in adjacent normal tissues (1.253 ±0.1438) with signi?cant difference (P < 0. 01). The expression of KIF 18 A protein located mainly in cytoplasm. Positive rate of KIF 18 A expression in colorectal cancer tissues was 80% (74/92), which was obviously higher than that in normal colorectal tissues ( 15 %,3 / 20 ) with significant difference (χ 2 = 32 . 741 , P < 0 . 01 ). The analysis on clinicopathological parameters suggested that the expression level of KIF18A in cancer tissues was associated with invasion depth (χ2=15.031, P<0.01), lymph node metastasis (χ2= 6.064, P<0.05) and pTNM staging (χ2=6.546, P<0.05). The overall 3-year survival of colorectal cancer patients with high levels of KIF18A expression was lower than that of patients with low levels of KIF18A expression (54.1% vs 100%, χ2=15.326, P<0.01). Univariate Cox regression analysis showed that tumor size (P<0.05), degree of differentiation (P<0.01), depth of tumor invasion (P<0.01), lymph node metastasis (P < 0 . 01), pTNM staging (P < 0 . 01) and KIF18A expression level (P < 0 . 01) were associated with the prognosis of colorectal cancer patients. Multivariate Cox regression analysis indicated that the level of KIF18A expression was independent factor impacting the survival of colorectal cancer patients (HR = 11. 419, 95% CI:1.504~86.684, P<0.05). Conclusion The high expression of KIF18A in colorectal cancer suggests that KIF18A may be associated with poor prognosis of patients with colorectal cancer and may be a potential marker for diagnosis, treatment and prognosis of colorectal cancer. |
| Key words: Kinesin family18A colorectal neoplasms prognosis |
