| 摘要: |
| 目的：探讨胃癌组织中X染色体连锁锌指蛋白（ZFX）的表达，评价其与患者临床病理特征及预后的关系。
方法：选取2013年1月—2015年12月经手术切除的胃癌组织标本，并收集其癌旁组织标本作为对照组。采用qRT-PCR方法检测43例胃癌组织及其对应癌旁组织中ZFX mRNA的表达，免疫组织化学（IHC）方法进一步检测114例胃癌及其癌旁组织中ZFX蛋白的表达情况，观察并探讨其与临床病理参数之间的关系，采用Kaplan-Meier对胃癌患者生存情况进行分析。结果：qRT-PCR结果显示ZFX mRNA在胃癌组织中的表达异常升高，明显高于癌旁组织；进一步IHC结果表明，ZFX蛋白的表达位于细胞核，胃癌组织中的表达阳性率约为76.3％，而癌旁组织中的阳性率仅有19.3％，差异有统计学意义（P<0.01）。进一步分析显示，胃癌组织中ZFX蛋白的表达与患者的年龄、性别、肿瘤部位及TNM分期无关（P>0.05），而与肿瘤分化程度、淋巴结转移、肿瘤的浸润深度密切相关（P<0.05）。Kaplan-Meier生存曲线显示ZFX蛋白阴性表达组的总生存期高于阳性表达组（P<0.05）。结论：ZFX在胃癌组织中于基因水平和蛋白水平均呈现异常高表达，并与患者临床病理参数和预后密切相关，提示ZFX参与胃癌的发生发展，有望成为胃癌潜在的预后指标。 |
| 关键词: 胃癌 ZFX 临床病理参数 预后 |
| DOI：10.3969/j.issn.1007-6948.2019.03.005 |
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| 基金项目: |
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| Expression of ZFX Protein and its Relationship with Pathology and Prognosis in Gastric Cancer |
| CUI Ji-fang, WANG Hong-lei, ZHAO Yong-jie, et al. |
| Department of Gastroenterology, Tianjin People' s Hospital, Tianjin (300122), China |
| Abstract: |
| Objective To explore the relationship between the expression of zinc finger X-linked protein (ZFX) and clinicopathological parameters and prognosis in gastric cancer (GC). Methods Surgical GC tissue samples and paired paracancerous tissues from patients undergoing resection were selected from January 2013 to December 2015. The mRNA expression of ZFX in 43 cases of GC tissues\ and corresponding paracancerous tissue were determined by quantitative real time-polymerase chain reaction (qRT-PCR). Immunohistochemistry (IHC) technology was adopted to measure the expression of ZFX protein in 114 cases of GC tissue and corresponding paracancerous tissue. The relationship between ZFX expression and clinicopathological parameters were explored. Kaplan-Meier was used to further analyze the overall survival (OS) of GC patients. Results qRT-PCR results showed that the mRNA level of ZFX was significantly up-regulated in GC tissues than that in paired paracancerous tissues. As for the IHC consequence, ZFX protein was located in cell nucleus. The positive expression rate of ZFX protein in GC group was about 76.3%, while that in the control group was only 19.3%, the difference was statistically significant (P<0.01). The expression of ZFX protein was closely related to tumor differentiation, lymph node metastasis and tumor infiltration depth (P<0.05), but not associated with age, gender, tumor location and TNM stage (P>0.05). Kaplan-Meier survival curve showed that OS in ZFX negative expression group were higher than those in the ZFX positive group (P<0.05). Conclusion ZFX is highly expressed in GC tissues both on gene level and protein level and closely related to clinicopathological parameters and prognosis of patients, which strongly suggests that ZFX is related to the initial and development of gastric cancer, and it is considered as a potential predictor for prognosis of GC patients. |
| Key words: Gastric cancer ZFX clinicopathological parameters prognosis |
