Effects of Xinlikang Capsule on Survival Time of Lewis Lung Cancer Model Mice and Its Mechanism of Action
WANG Qing,QUE Zu-jun,TIAN Jian-hui
Institute of Oncology, Shanghai Academy of Traditional Chinese Medicine, Shanghai (200032), China
Abstract:
Objective To study the effects of Xinlikang Capsule on survival time and life quality of the model mice of pulmonary cancer metastasis of lung cancer, and explore the mechanism. Methods Mouse model of pulmonary cancer metastasis of Lewis lung carcinoma (LLC) was established through tail intravenous injection, and were divided into saline group, low-dose Xinlikang group (0.08 g/mL) and high-dose Xinlikang group (0.40 g/mL), 10 mice in each group. The activity, food intake, hair glossiness and survival time were observed. Besides, the effects of Xinlikang on tumor-related signal transducer and activator of transcription 3 (STAT 3 ) and nuclear factor-κB (NFκB) signal pathways were analyzed with luciferase assay. Results The mice of saline group showed as depression, drowsiness, low glossy hair, low activity, little food intake and drinking, which was similar to mice in the low-dose Xinlikang group, but the mice in high–dose Xinlikang group showed as good spirit, glossy hair, more activity and more food intake and drinking. The median survival time was 17 d in saline group, 17.5 d in low concentration group, and 21 d in high-dose Xinlikang group which was signi?cantly more than that in saline group (P < 0 . 05 ). Xinlikang Capsule significantly inhibited the STAT 3 and NFκB signal pathways. Conclusion Xinlikang Capsule can prolong the survival time and improve the quality of life of mouse model with pulmonary cancer metastasis of lung cancer, maybe partly through inhibiting STAT 3 and NFκB signal pathways.
