| 摘要: |
| 观察参黄合剂对大鼠瘘管创面组织中血管生成及内皮细胞凋亡的影响。方法：选用180只雄性SD大鼠用于瘘管术后模型，造模成功后，按随机数字表法分为空白对照组、空白模型组、模型对照组、生理盐水组、高锰酸钾组、参黄合剂组等6组，每组30只。分别于术后第l、7、14 d从各组取出10只大鼠处死取标本，观察各组大鼠创面肉芽形态变化及血管内皮细胞凋亡情况。结果：术后1 d、7 d和14 d空白对照组镜下毛细血管及凋亡细胞无明显变化：其余各组术后1 d可见凋亡细胞、毛细血管及大量渗出液及炎性细胞，术后7 d、14 d可见大量由内皮细胞增生形成的实性细胞索及扩张的毛细血管，在毛细血管周围有许多新生的成纤维细胞，凋亡细胞、渗出液及炎性细胞逐渐减少。在术后第7 d时参黄合剂组VEGF mRNA（13.64±3.19）开始增加，凋亡指数（1.92±0.15）开始下降，均达到高速增长期；第14 d参黄合剂组VEGF mRNA（18.48±3.18）继续上升、凋亡指数（1.48±0.16）继续下降，速度放缓。治疗7 d时，参黄合剂组大鼠创面肉芽组织中VEGF mRNA及凋亡指数与空白对照组VEGF mRNA（7.37±1.32）、凋亡指数（2.42±0.16），生理盐水组VEGF mRNA（7.76±1.05）、凋亡指数（2.53±0.17）及高锰酸钾组VEGF mRNA（10.53±1.24）、凋亡指数（2.16±0.17）比较，差异具有统计学意义（P<0.05）。治疗14 d时，参黄合剂组创面VEGF mRNA及凋亡指数与空白对照组VEGF mRNA（9.46±1.25）、凋亡指数（2.15± 0.14），生理盐水组VEGF mRNA（9.47±1.73）、凋亡指数（2.22± 0.15）及高锰酸钾组VEGF mRNA（14.65±1.45）、凋亡指数（1.76±0.11）比较，差异具有统计学意义(P<0.05)。结论：参黄合剂促进创面肉芽组织中血管生成，抑制了血管内皮细胞的凋亡，调节了VEGF mRNA的表达，加速创面修复。 |
| 关键词: 参黄合剂 瘘管 血管生成 内皮细胞凋亡 大鼠 |
| DOI：10.3969/j.issn.1007-6948.2019.01.011 |
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| 基金项目:安徽省卫生计生委中医药科研课题项目（2014zy53）；安徽省卫生和计划生育委员会科研计划项目（全科医学临床科研课题）（2016QK056） |
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| Effect of Shenhuang Mixture on Blood Vessel and Endothelial Cells of Rat Fistula Wound |
| WANG Chuan-si,XIE Yi-xiang,YU Qing-sheng,YAO Lei,HUANG Hongwu,WANG Yongsen,WANG Xiaolong |
| Department of Anorectal Surgery, The Affiliated Lu' an Hospital of Anhui Medical University, Lu' an (237005), China;Department of General Surgery, the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei 230031, China |
| Abstract: |
| Objective To observe the effect of Shenhuang Mixture on angiogenesis and endothelial cell apoptosis in rat fistula wound tissue. Methods Male Sprague-Dawley rats (180 rats) were used for the post-fistula model. After the model was established successfully, the rats were divided into blank control group, blank model group, model control group, saline group, potassium permanganate group and Shenhuang Mixture group(n=30). Ten rabbits were sacrificed on day 1, 7 and 14 after operation respectively from each group to observe the morphological changes of granulation and apoptosis of vascular endothelial cells. Result There was no significant change of capillary and apoptotic cells in the control group on day 1, 7 and 14 after operation. Apoptotic cells, capillaries, exudates and inflammatory cells were observed on day 1 after operation in other groups. On day 7 and 14 after operation, a large number of solid cords and dilated capillaries formed by endothelial cell proliferation were observed. There were many new fibroblasts around the capillaries. Apoptotic cells, exudates and inflammatory cells gradually decreased. On day 7 after operation, the expression of VEGF mRNA (13.64±3.19) and the apoptotic index (1.92±0.15) in Shenhuang Mixture group began to decrease, all of which reached the high growth phase. On day 14, the VEGF mRNA expression in Shenhuang Mixture group was (18.48±3.18), continued to rise, the apoptotic index (1.48±0.16) continued to decline, slowing down. On day 7 after treatment, VEGF mRNA and apoptosis index in granulation tissue of Shenhuang Mixture group were significantly higher than VEGF mRNA (7.37±1.32) and apoptotic index (2.42±0.16) in blank control group, VEGF mRNA (7.76±1.05) and apoptotic index (2.53±0.17) in normal saline group, and VEGF mRNA (10.53±1.24) and apoptotic index (2.16±0.17) in potassium permanganate group (P <0.05). On day 14 of treatment, VEGF mRNA and apoptosis index in Shenhuang Mixture group were significantly higher than VEGF mRNA (9.46±1.25) and apoptotic index (2.15±0.14) in blank control group, VEGF mRNA (9.47±1.73) and apoptotic index (2.22±0.15) in normal saline group, and VEGF mRNA (14.65±1.45) and apoptotic index (1.76±0.11) in potassium permanganate group. The difference was statistically significant (P<0.05). Conclusion The effect of Shenhuang Mixture is to promote angiogenesis in granulation tissue, inhibit the apoptosis of vascular endothelial cells, regulate the expression of VEGF mRNA, and accelerate the wound repair. |
| Key words: Shenhuang Mixture fistula angiogenesis endothelial cell apoptosis rats |
