Effect of Shenhuang Mixture on Blood Vessel and Endothelial Cells of Rat Fistula Wound
WANG Chuan-si,XIE Yi-xiang,YU Qing-sheng,YAO Lei,HUANG Hongwu,WANG Yongsen,WANG Xiaolong
Department of Anorectal Surgery, The Affiliated Lu' an Hospital of Anhui Medical University, Lu' an (237005), China;Department of General Surgery, the First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei 230031, China
Abstract:
Objective To observe the effect of Shenhuang Mixture on angiogenesis and endothelial cell apoptosis in rat fistula wound tissue. Methods Male Sprague-Dawley rats (180 rats) were used for the post-fistula model. After the model was established successfully, the rats were divided into blank control group, blank model group, model control group, saline group, potassium permanganate group and Shenhuang Mixture group(n=30). Ten rabbits were sacrificed on day 1, 7 and 14 after operation respectively from each group to observe the morphological changes of granulation and apoptosis of vascular endothelial cells. Result There was no significant change of capillary and apoptotic cells in the control group on day 1, 7 and 14 after operation. Apoptotic cells, capillaries, exudates and inflammatory cells were observed on day 1 after operation in other groups. On day 7 and 14 after operation, a large number of solid cords and dilated capillaries formed by endothelial cell proliferation were observed. There were many new fibroblasts around the capillaries. Apoptotic cells, exudates and inflammatory cells gradually decreased. On day 7 after operation, the expression of VEGF mRNA (13.64±3.19) and the apoptotic index (1.92±0.15) in Shenhuang Mixture group began to decrease, all of which reached the high growth phase. On day 14, the VEGF mRNA expression in Shenhuang Mixture group was (18.48±3.18), continued to rise, the apoptotic index (1.48±0.16) continued to decline, slowing down. On day 7 after treatment, VEGF mRNA and apoptosis index in granulation tissue of Shenhuang Mixture group were significantly higher than VEGF mRNA (7.37±1.32) and apoptotic index (2.42±0.16) in blank control group, VEGF mRNA (7.76±1.05) and apoptotic index (2.53±0.17) in normal saline group, and VEGF mRNA (10.53±1.24) and apoptotic index (2.16±0.17) in potassium permanganate group (P <0.05). On day 14 of treatment, VEGF mRNA and apoptosis index in Shenhuang Mixture group were significantly higher than VEGF mRNA (9.46±1.25) and apoptotic index (2.15±0.14) in blank control group, VEGF mRNA (9.47±1.73) and apoptotic index (2.22±0.15) in normal saline group, and VEGF mRNA (14.65±1.45) and apoptotic index (1.76±0.11) in potassium permanganate group. The difference was statistically significant (P<0.05). Conclusion The effect of Shenhuang Mixture is to promote angiogenesis in granulation tissue, inhibit the apoptosis of vascular endothelial cells, regulate the expression of VEGF mRNA, and accelerate the wound repair.
